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Microorganisms for producing putrescine and process for producing putrescine using them
US9657264B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9657264-B2 |
| Application number | US-201515306753-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 24, 2015 |
| Priority date | Apr 25, 2014 |
| Publication date | May 23, 2017 |
| Grant date | May 23, 2017 |
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- Title
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Abstract
Official abstract text for this publication.
The present invention relates to a recombinant microorganism capable of producing putrescine at high yield due to inactivated activity of a protein having an amino acid sequence represented by SEQ ID NO: 2 in the microorganism, and a method of producing putrescine using the microorganism.
First claim
Opening claim text (preview).
The invention claimed is: 1. An isolated microorganism of genus Corynebacterium having putrescine productivity, wherein a protein having an amino acid sequence represented by SEQ ID NO: 2 is inactivated. 2. The microorganism of genus Corynebacterium having putrescine productivity according to claim 1 , wherein an activity of ornithine decarboxylase (ODC) is further introduced into the microorganism. 3. The microorganism of genus Corynebacterium having putrescine productivity according to claim 2 , wherein the ODC has an amino acid sequence represented by SEQ ID NO: 10. 4. The microorganism of genus Corynebacterium having putrescine productivity according to claim 1 , wherein acetyltransferase activity is further inactivated in the microorganism. 5. The microorganism of genus Corynebacterium having putrescine productivity according to claim 4 , wherein the acetyltransferase comprises an amino acid represented by SEQ ID NO: 15 or 16. 6. The microorganism of genus Corynebacterium having putrescine productivity according to claim 1 , wherein the microorganism is Corynebacterium glutamicum. 7. A method of producing putrescine, comprising: culturing a microorganism of genus Corynebacterium having putrescine productivity wherein a protein having an amino acid sequence represented by SEQ ID NO: 2 is inactivated, in a culture medium; and separating putrescine from the cultured microorganism or the culture medium obtained from the above step. 8. The method of producing putrescine according to claim 7 , wherein the microorganism of genus Corynebacterium is Corynebacterium glutamicum.
Assignees
Inventors
Classifications
Amines; Imines · CPC title
- C12N1/20Primary
Bacteria; Culture media therefor · CPC title
Ornithine decarboxylase (4.1.1.17) · CPC title
from Corynebacterium (G) · CPC title
Corynebacterium · CPC title
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- What does patent US9657264B2 cover?
- The present invention relates to a recombinant microorganism capable of producing putrescine at high yield due to inactivated activity of a protein having an amino acid sequence represented by SEQ ID NO: 2 in the microorganism, and a method of producing putrescine using the microorganism.
- Who is the assignee on this patent?
- Cj Cheiljedang Corp
- What technology area does this patent fall under?
- Primary CPC classification C12N1/20. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue May 23 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).