Probiotic arginolytic oral compositions and methods of making and using probiotic arginolytic oral compositions

Patent metadata

Field	Value
Publication number	US-9655839-B2
Application number	US-201414767181-A
Country	US
Kind code	B2
Filing date	Feb 12, 2014
Priority date	Feb 14, 2013
Publication date	May 23, 2017
Grant date	May 23, 2017

Abstract

The present disclosure provides probiotic arginolytic oral compositions, methods of making probiotic arginolytic oral compositions, and methods of using probiotic arginolytic oral compositions to increase arginolytic activity in the oral cavity and/or to treat and/or prevent caries.

First claim

The invention claimed is:

1. A method for making a mixture of arginolytic bacterial strains for oral use, the method comprising:
(a) obtaining a mixture of bacterial strains isolated from oral samples;
(b) isolating and identifying arginolytic bacterial strains capable of producing ammonia via the arginine deiminase system (ADS);
(c) conducting one or more separate assays to identify arginolytic bacterial strains capable of expressing ADS activity in at least one of the following assay conditions: in the absence of environmental arginine, in the presence of glucose, in a non-acidic pH, in aerobic conditions, and in the presence of at least one bacterial strain associated with dental caries;
(d) selecting at least two different isolated arginolytic bacterial strains identified in step (c) to prepare a mixture of arginolytic bacteria; and
(e) preparing a mixture of the at least two isolated arginolytic bacterial strains, wherein the mixture expresses ADS activity in at least two of the conditions, and wherein the mixture does not include any strains of Streptococcus mutans.

2. The method of claim 1 , wherein the ability of the bacterial strain to express ADS activity in any of said assay conditions is determined with respect to the ADS activity level of S. gordonii DL1 under the same conditions, wherein an ADS activity level about the same or higher than the activity of S. gordonii DL1 under the same conditions indicates the strain expresses ADS activity under the conditions.

3. The method of claim 1 , wherein the bacterial strain associated with dental caries is Streptococcus mutans.

4. The method of claim 1 , further comprising including in the mixture one or more compounds capable of increasing the ADS activity of the bacterial strains with respect to the ADS activity of the strains in the absence of the compounds.

5. The method of claim 4 , wherein the one or more compounds to increase the ADS activity of the bacterial strains are selected from the group consisting of: galactose, arginine, and an acidic compound.

Assignees

• Univ Florida

Inventors

• Fagerberg Marcelle Matos Nascimento
• Burne Robert A

Classifications

• G01N2333/315

  from Streptococcus (G), e.g. Enterococci · CPC title

• A61Q11/00

  Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses · CPC title

• A61K8/99 Primary

  from microorganisms other than algae or fungi, e.g. protozoa or bacteria · CPC title

• C12Q1/14

  Streptococcus; Staphylococcus · CPC title

• A61K31/7004

  Monosaccharides having only carbon, hydrogen and oxygen atoms · CPC title