Enhanced spray formation for liquid samples

Patent metadata

Field	Value
Publication number	US-9653276-B2
Application number	US-201414910032-A
Country	US
Kind code	B2
Filing date	Aug 5, 2014
Priority date	Aug 7, 2013
Publication date	May 16, 2017
Grant date	May 16, 2017

Abstract

Methods and systems for generating ions from a liquid sample for mass spectrometry are provided herein. In various aspects, the methods and systems can enhance the break-up of a jet of the liquid sample upon injection into an ionization chamber. In some aspects, methods and systems perturb the liquid sample prior to discharge to increase the internal energy of the sample so as to enhance the formation of liquid droplets when the liquid sample is injected into the ionization chamber.

First claim

What is claimed is:

1. An apparatus for generating ions for analysis by a mass spectrometer, comprising:
an ion source housing defining an ion source chamber, the ion source chamber configured to be in fluid communication with a sampling orifice of a mass spectrometer;
a conduit having an inlet end for receiving a liquid sample and an outlet end for discharging the liquid sample into the ion source chamber such that the discharged liquid forms a sample plume, the sample plume comprising a plurality of liquid droplets;
means for mechanically perturbing the liquid sample flowing within the conduit so as to enhance the formation of liquid droplets when the liquid sample is discharged from the outlet end into the ion source chamber;
wherein the outlet end of the conduit extends through a second conduit configured to supply a nebulizer gas at the outlet of the first conduit; and
means for ionizing one or more analytes contained within the liquid droplets wherein the means for mechanically perturbing the liquid sample comprises an oscillating diaphragm in fluid communication with the liquid sample within the conduit.

2. The apparatus of claim 1 , wherein the conduit comprises a capillary tube.

3. The apparatus of claim 2 , wherein the nebulizer gas has a flow rate in a range from about 0.1 L/min. to about 20 L/min.

4. The apparatus of claim 1 , wherein the nebulizer gas has a flow rate such that a mass ratio of the nebulizer gas to the liquid sample being nebulized is less than about 50 over a liquid flow range of about 10 μL/min to about 10 mL/min; and optionally
wherein the nebulizer gas has a flow rate such that the mass ratio is less than about 30 over the liquid flow range of 10 μL/min to 10 mL/min.

5. The apparatus of claim 1 , wherein the outlet end of the conduit comprises a nozzle.

6. The apparatus of claim 1 , wherein said means for perturbing the liquid sample comprises means for increasing the internal energy of the liquid sample; optionally
wherein said means for perturbing the liquid sample comprises means for generating pressure waves within the liquid sample; and optionally
wherein said pressure waves are configured to generate cavitation bubbles within the liquid sample.

7. The apparatus of claim 1 ,
wherein the diaphragm oscillates at a frequency less than about 20 kHz; and optionally
wherein the frequency is less than about 1000 Hz.

8. The apparatus of claim 6 , wherein said means for perturbing the liquid sample comprises an ultrasonic transducer.

9. The apparatus of claim 1 , wherein said means for perturbing the liquid sample is configured to generate cavitation bubbles within the liquid sample.

10. The apparatus of claim 1 , wherein said means for perturbing the liquid sample comprises flow restrictions in said conduit and optionally wherein said means for perturbing the liquid sample comprises baffles within said conduit.

11. The apparatus of claim 1 , wherein said means for perturbing the liquid sample is configured to increase a liquid/gas phase heterogeneity of the liquid sample within the conduit, wherein the liquid sample comprises a substantially homogenous liquid phase at the inlet end of the conduit.

12. The apparatus of claim 1 , further comprising a heater for heating the liquid sample flowing in the conduit.

13. A method of generating ions for analysis by a mass spectrometer, comprising:
receiving a liquid sample at an inlet end of a conduit from a sample source;
transporting the liquid sample from the inlet end of the conduit to an outlet end of the conduit;
mechanically perturbing the liquid sample while being transported within the conduit and prior to being discharged in the chamber;
wherein the conduit outlet extends through a second conduit configured to supply a nebulizer gas at the outlet of the first conduit;
discharging the entire liquid sample from an outlet end of the conduit to an ion source chamber such that the discharged liquid forms a sample plume comprising a plurality of liquid droplets; and
ionizing an analyte contained within the liquid droplets prior to entering a sampling orifice of a mass spectrometer in fluid communication with the ion source chamber wherein said means for perturbing the liquid sample is configured to inject gas within the liquid sample and further comprises means for mixing the liquid sample following gas injection to distribute gas bubbles within the liquid sample.

14. The method of claim 13 , wherein perturbing the liquid sample comprises increasing the internal energy of the liquid sample and optionally
wherein perturbing the liquid sample comprises generating pressure waves within the liquid sample in the conduit.

15. The method of claim 13 , wherein perturbing the liquid sample comprises generating cavitation bubbles within the liquid sample in the conduit.

16. The method of claim 13 , wherein mechanically perturbing the sample comprises increasing a liquid/gas phase heterogeneity of the liquid sample within the conduit, wherein the liquid sample comprises a substantially homogenous liquid phase at the inlet end of the conduit.

17. The method of claim 13 , further comprising heating the liquid sample within the conduit.

Assignees

• Dh Technologies Dev Pte Ltd

Inventors

• Covey Thomas
• Kovarik Peter

Classifications

• H01J49/165 Primary

  Electrospray ionisation · CPC title

• H01J49/045

  with means for using a nebulising gas, i.e. pneumatically assisted · CPC title