Nano-fluidic field effective device to control DNA transport through the same

Patent metadata

Field	Value
Publication number	US-9651518-B2
Application number	US-201414543212-A
Country	US
Kind code	B2
Filing date	Nov 17, 2014
Priority date	Jun 22, 2010
Publication date	May 16, 2017
Grant date	May 16, 2017

Abstract

The present invention provides a nano-fluidic field effective device. The device includes a channel having a first side and a second side, a first set of electrodes adjacent to the first side, a second set of electrodes adjacent to the second side, a control unit for applying electric potentials to the electrodes and a fluid within the channel containing a charge molecule. The first set of electrodes is disposed such that application of electric potentials produces a spatially varying electric field that confines a charged molecule within a predetermined area of said channel. The second set of electrodes is disposed such that application of electric potentials relative to the electric potentials applied to the first set of electrodes creates an electric field that confines the charged molecule to an area away from the second side of the channel.

First claim

What is claimed is:

1. A method of controlling charged molecules in a nano-fluidic field effective device having a channel, the method comprising:
applying a first electric field in a direction of a flow axis of said channel in said nano-fluidic field effective device to drive a first charged molecule and a second charged molecule into said channel;
applying a second electric field in a direction parallel to a horizontal axis perpendicular to said flow axis by generating a first potential difference between a first tooth of a first comb-shaped electrode of a first pair of interdigitated combed-shaped electrodes and a second tooth of a second comb-shaped electrode of the first pair of interdigitated combed-shaped electrodes to confine said first charged molecule to a first subslot in the channel; and
applying a third electric field in a direction parallel to the second electric field by generating a second potential difference between a third tooth of the first comb-shaped electrode and a fourth tooth of the second combed-shaped electrode to confine the second charged molecule to a second subslot in the channel, wherein
the second subslot is parallel to the first subslot, and
the third tooth is located in a different area of the first comb-shaped electrode than the first tooth;

driving the first charged molecule through the first subslot using a fourth electric field having a direction parallel to the first electric field and generated by a third potential difference between the first comb-shaped electrode and a third comb-shaped electrode.

2. The method of controlling charged molecules of claim 1 , wherein said second electric field is spatially varying.

3. The method of controlling charged molecules of claim 2 , further comprising:
loading said first charged molecule into said channel, wherein said loading is carried out by applying different electric potentials to two electrodes positioned inside chambers on opposite ends of said channel.

4. The method of controlling charged molecules of claim 2 , wherein said first charged molecule is single stranded DNA, the method further comprising:
sensing bases of said first charged molecule.

5. The method of controlling charged molecules of claim 4 , further comprising:
applying a fifth electric field to stretch said first charged molecule such that a spacing between neighboring bases is increased.

6. The method of controlling charged molecules of claim 2 , wherein said first charged molecule is a long chain polymer.

7. The method of controlling charged molecules of claim 2 , wherein said first charged molecule is single-strand DNA.

8. The method of controlling charged molecules of claim 1 , wherein said step of applying a first electric field in a direction of a flow axis is carried out by applying electric potentials to neighboring electrodes disposed such that said first electric field is applied in said direction of said flow axis.

9. The method of controlling charged molecules of claim 1 , wherein said first electric field applied in said direction of said flow axis of said channel is time-dependent.

10. The method of controlling charged molecules of claim 1 , wherein a dimension of both of said first tooth and said second tooth is selected so that said second electric field prevents said first charged molecule from meandering in said first subslot.

11. The method of controlling charged molecules of claim 10 , wherein said second electric field is time-dependent.

12. The method of controlling charged molecules of claim 1 , wherein said second electric field has a component applied in a vertical direction, said vertical direction perpendicular to both said flow axis and said horizontal axis.

13. The method of controlling charged molecules of claim 12 , wherein said component of said second electric field applied in said vertical direction is time-dependent.

14. The method of controlling charged molecules of claim 1 , wherein said second electric field is applied in a vertical direction and a horizontal direction.

Assignees

• IBM

Inventors

• Afzali-Ardakani Ali
• Harrer Stefan
• Luan Binquan
• Martyna Glenn J
• Newns Dennis M
• Peng Hongbo
• Polonsky Stanislav
• Rossnagel Stephen
• Stolovitzky Gustavo

Classifications

• B82Y15/00

  Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors · CPC title

• G01N27/44791

  Microapparatus (sample containers with integrated microfluidic structures B01L3/5027) · CPC title

• G01N33/48721

  Investigating individual macromolecules, e.g. by translocation through nanopores (Coulter counters in general G01N15/12; fabrication methods for nanoscale apertures B81B1/00; sequencing of nucleic acids C12Q1/68) · CPC title

• B01L2400/0421

  electrophoretic flow · CPC title

• G01N27/44756 Primary

  Apparatus specially adapted therefor · CPC title