Polypeptides having glucoamylase activity and polynucleotides encoding same

Patent metadata

Field	Value
Publication number	US-9650620-B2
Application number	US-201214241344-A
Country	US
Kind code	B2
Filing date	Aug 24, 2012
Priority date	Aug 26, 2011
Publication date	May 16, 2017
Grant date	May 16, 2017

Abstract

Provided are isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.

First claim

The invention claimed is:

1. A composition comprising a polypeptide having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4, wherein the polypeptide has glucoamylase activity.

2. A composition comprising an alpha-amylase and a polypeptide having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4, wherein the polypeptide has glucoamylase activity.

3. A process of producing a fermentation product from starch-containing material comprising the steps of:
(a) liquefying starch-containing material;
(b) saccharifying the liquefied material to form a saccharified material; and
(c) fermenting the saccharified material with a fermenting organism;

wherein step (b) is carried out using at least a glucoamylase having at least at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4.

4. A process of producing a fermentation product from starch-containing material, comprising the steps of:
(a) saccharifying starch-containing material with an alpha amylase and a glucoamylase having at least at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4, at a temperature below the initial gelatinization temperature of said starch-containing material to form saccharified material; and
(b) fermenting the saccharified material with a fermenting organism.

5. A recombinant nucleic acid construct or recombinant expression vector comprising a polynucleotide encoding a polypeptide having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4 operably linked to one or more control sequences that direct the production of the polypeptide in an expression host.

6. A recombinant host cell comprising a polynucleotide encoding a polypeptide having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4 operably linked to one or more control sequences that direct the production of the polypeptide.

7. A method of producing a polypeptide of having at least at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4, comprising:
(a) cultivating a recombinant host cell comprising a polynucleotide encoding a polypeptide having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4 under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.

8. A transgenic plant, plant part or plant cell transformed with a polynucleotide encoding a polypeptide having at least at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4.

9. A method of producing a polypeptide having at least at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4, comprising:
(a) cultivating the transgenic plant or plant cell of claim 8 under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.

10. The recombinant host cell of claim 6 , wherein the host cell is Candida, Hansenula, Kluyveromyces, Pichia, Saccharomyces, Schizosaccharomyces , or Yarrowia.

11. The recombinant host cell of claim 10 , wherein the host cell is Saccharomyces carlsbergensis, Saccharomyces cerevisiae, Saccharomyces diastaticus, Saccharomyces douglasii, Saccharomyces kluyveri, Saccharomyces norbensis , or Saccharomyces oviformis.

12. The recombinant host cell of claim 10 , wherein the host cell is Saccharomyces cerevisiae.

13. A method of producing a polypeptide of having at least at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4, comprising:
(a) cultivating a recombinant host cell comprising a polynucleotide encoding a polypeptide having at least at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4 under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide, wherein the polypeptide has glucoamylase activity.

14. The method of claim 13 , wherein the polypeptide has at least 96% sequence identity to the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4.

15. The method of claim 13 , wherein the polypeptide has at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4.

16. The method of claim 13 , wherein the polypeptide has at least 98% sequence identity to the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4.

17. The method of claim 13 , wherein the polypeptide has at least 99% sequence identity to the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4.

18. The recombinant host cell of claim 6 , wherein the polynucleotide encodes a polypeptide having at least at least 99% sequence identity to the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4.

Assignees

• Sun tianqi
• Li Ming
• Novozymes As

Inventors

• Sun tianqi
• Li Ming

Classifications

• C12P19/20

  produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose · CPC title

• C12Y302/01003

  Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase · CPC title

• Y02P20/52

  using catalysts, e.g. selective catalysts · CPC title

• C12N9/2428 Primary

  Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase · CPC title

• C12P19/02

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title