Self-assembled nanostructures

Patent metadata

Field	Value
Publication number	US-9650421-B2
Application number	US-201214342245-A
Country	US
Kind code	B2
Filing date	Aug 31, 2012
Priority date	Sep 2, 2011
Publication date	May 16, 2017
Grant date	May 16, 2017

Abstract

The present disclosure is directed to the preparation of nanostructures by the encapsulation of a charged compound with individual self-assembled unit nano structures.

First claim

What is claimed is:

1. A peptide segment comprising a first domain, a second domain and a third domain, wherein:
(a) the first domain is selected from the group consisting of spermine, oligoethyleneimine, polyethyleneimine, protamine, and a histone, and is positioned at a first terminus of the second domain;
(b) the second domain comprises an amino acid sequence of SEQ ID NO: 1, and is positioned between the first domain and the third domain; and
(c) the third domain is a polyethylene glycol (PEG), and is positioned at a second terminus of the second domain,
wherein a plurality of peptide segments have the ability to self assemble to form a capsomer.

2. The peptide segment of claim 1 , comprising at least one non-naturally occurring amino acid.

3. The peptide segment of claim 1 , wherein the PEG has an average molecular weight of about 2 kilodaltons to about 100 kilodaltons.

4. The peptide segment of claim 1 , wherein the PEG has an average molecular weight of about 2 kilodaltons to about 5 kilodaltons.

5. A capsomer comprising a plurality of peptide segments, each according to claim 1 , wherein the capsomer is formed by the self-assembly of the plurality of peptide segments, and wherein a plurality of capsomers have the ability to self assemble to form a nanostructure.

6. A nanostructure comprising a plurality of capsomers, each capsomer according to claim 5 , and further comprising a charged compound.

7. The nanostructure of claim 6 , comprising at least two different charged compounds.

8. The nanostructure of claim 6 , wherein each capsomer is identical.

9. The nanostructure of claim 6 , comprising at least two capsomers that are not identical.

10. The nanostructure of claim 6 , wherein the charged compound is a nucleic acid.

11. The nanostructure of claim 10 , wherein the nucleic acid is double stranded.

12. The nanostructure of claim 6 , wherein the charged compound is encapsulated by the plurality of capsomers.

13. The nanostructure of claim 6 , which has an electronic property selected from the group consisting of semiconductivity, conductivity and electrochromism.

14. The nanostructure of claim 6 , which is a monodisperse filamentous structure.

Assignees

• Stupp Samuel I
• Ruff Yves
• Univ Northwestern

Inventors

• Stupp Samuel I
• Ruff Yves

Classifications

• A61K47/69

  the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit · CPC title

• C07K2319/20

  containing a tag with affinity for a non-protein ligand · CPC title

• A61K47/60

  the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol · CPC title

• C07K14/00

  Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof · CPC title

• A61K47/6455

  Polycationic oligopeptides, polypeptides or polyamino acids, e.g. for complexing nucleic acids · CPC title