Mass spectrometer

Patent metadata

Field	Value
Publication number	US-9646813-B2
Application number	US-201514689161-A
Country	US
Kind code	B2
Filing date	Apr 17, 2015
Priority date	Dec 7, 2004
Publication date	May 9, 2017
Grant date	May 9, 2017

Abstract

A mass spectrometer is disclosed comprising a first quadrupole rod set mass filter, a collision cell, an ion mobility spectrometer or separator, an ion guide or collision cell arranged downstream of the ion mobility spectrometer or separator, a second quadrupole rod set mass filter and an ion detector.

First claim

The invention claimed is:

1. A mass spectrometer comprising:
an ion mobility spectrometer or separator for temporally separating parent or precursor ions;
a mass filter arranged downstream of said ion mobility spectrometer or separator for mass filtering ions, wherein the mass filter is arranged and configured to transmit different ions at different times;
a collision, fragmentation or reaction device for fragmenting or reacting parent or precursor ions to produce fragment or product ions; and
a mass analyser.

2. A mass spectrometer as claimed in claim 1 , wherein the collision, fragmentation or reaction device is arranged downstream of said mass filter; and the mass analyser is arranged downstream of the collision, fragmentation or reaction device for mass analysing the fragment or product.

3. A mass spectrometer as claimed in claim 1 , wherein said mass filter is scanned.

4. A mass spectrometer as claimed in claim 3 , wherein said mass filter is scanned in synchronism with said ion mobility spectrometer or separator.

5. A mass spectrometer as claimed in claim 1 , wherein said mass filter selectively transmits different precursor ions at different times.

6. A mass spectrometer as claimed in claim 5 , wherein said mass filter selectively transmits multiple different precursor ions during a single ion mobility separation period.

7. A mass spectrometer as claimed in claim 1 , wherein the mass filter selectively transmits parent or precursor ions based on their drift times through the ion mobility spectrometer or separator.

8. A mass spectrometer as claimed in claim 1 , wherein said mass analyser is a Time of Flight mass analyser.

9. A mass spectrometer as claimed in claim 1 , wherein said collision, fragmentation or reaction device is arranged and adapted to fragment ions by Collision Induced Dissociation.

10. A mass spectrometer as claimed in claim 1 , wherein said collision, fragmentation or reaction device is selected from the group consisting of: (i) a Surface Induced Dissociation (“SID”) fragmentation device; (ii) an Electron Transfer Dissociation fragmentation device; (iii) an Electron Capture Dissociation fragmentation device; (iv) an Electron Collision or Impact Dissociation fragmentation device; (v) a Photo Induced Dissociation (“PID”) fragmentation device; (vi) a Laser Induced Dissociation fragmentation device; (vii) an infrared radiation induced dissociation device; (viii) an ultraviolet radiation induced dissociation device; (ix) a nozzle-skimmer interface fragmentation device; (x) an in-source fragmentation device; (xi) an ion-source Collision Induced Dissociation fragmentation device; (xii) a thermal or temperature source fragmentation device; (xiii) an electric field induced fragmentation device; (xiv) a magnetic field induced fragmentation device; (xv) an enzyme digestion or enzyme degradation fragmentation device; (xvi) an ion-ion reaction fragmentation device; (xvii) an ion-molecule reaction fragmentation device; (xviii) an ion-atom reaction fragmentation device; (xix) an ion-metastable ion reaction fragmentation device; (xx) an ion-metastable molecule reaction fragmentation device; (xxi) an ion-metastable atom reaction fragmentation device; (xxii) an ion-ion reaction device for reacting ions to form adduct or product ions; (xxiii) an ion-molecule reaction device for reacting ions to form adduct or product ions; (xxiv) an ion-atom reaction device for reacting ions to form adduct or product ions; (xxv) an ion-metastable ion reaction device for reacting ions to form adduct or product ions; (xxvi) an ion-metastable molecule reaction device for reacting ions to form adduct or product ions; and (xxvii) an ion-metastable atom reaction device for reacting ions to form adduct or product ions.

11. A mass spectrometer as claimed in claim 1 , further comprising a voltage supply arranged and adapted to apply one or more voltages or potentials or one or more voltage or potential waveforms to electrodes forming said collision, fragmentation or reaction device in order to urge at least some ions along the axial length of said collision, fragmentation or reaction device.

12. A mass spectrometer as claimed in claim 1 , further comprising a transient DC voltage supply arranged and adapted to apply one or more transient DC voltages or potentials or one or more transient DC voltage or potential waveforms to electrodes forming said collision, fragmentation or reaction device in order to urge at least some ions along the axial length of said collision, fragmentation or reaction device.

13. A mass spectrometer as claimed in claim 1 , further comprising AC or RF voltage means arranged and adapted to apply two or more phase-shifted AC or RF voltages to electrodes forming said collision, fragmentation or reaction device in order to urge at least some ions along the axial length of said collision, fragmentation or reaction device.

14. A mass spectrometer as claimed in claim 1 , wherein said collision, fragmentation or reaction device is arranged and adapted to retain or confine or partition ions emerging from said ion mobility spectrometer or separator and to translate ions along the axial length of said collision, fragmentation or reaction device whilst substantially maintaining the order or fidelity in which ions emerge from said ion mobility spectrometer or separator.

15. A mass spectrometer as claimed in claim 1 , further comprising a control system arranged and adapted to switch or repeatedly switch said collision, fragmentation or reaction device between a relatively high fragmentation or reaction mode of operation wherein ions are substantially fragmented or reacted and a relatively low fragmentation or reaction mode of operation wherein substantially fewer or no ions are fragmented or reacted in said collision, fragmentation or reaction device.

16. A mass spectrometer as claimed in claim 1 , wherein said mass filter is a quadrupole rod set mass filter.

17. A method of mass spectrometry comprising:
temporally separating parent or precursor ions in an ion mobility spectrometer or separator;
mass filtering ions in a mass filter arranged downstream of said ion mobility spectrometer or separator, wherein the mass filter transmits different ions at different times;
fragmenting or reacting parent or precursor ions in a collision, fragmentation or reaction device to produce fragment or product ions; and
mass analysing said fragment or product ions.

Assignees

• Micromass Ltd

Inventors

• Bateman Robert Harold
• Giles Kevin

Classifications

• H01J49/022

  Circuit arrangements, e.g. for generating deviation currents or voltages (regulating electric or magnetic variables in general, e.g. current, magnetic field G05F); Components associated with high voltage supply (high voltage supply per se H02M) · CPC title

• H01J49/004 Primary

  Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn · CPC title

• H01J49/062

  Ion guides (linear ion traps performing mass selection H01J49/4225, mass filters H01J49/421) · CPC title

• H01J49/0045 Primary

  characterised by the fragmentation or other specific reaction · CPC title

• G01N27/622

  Ion mobility spectrometry · CPC title