System for detecting rare cells

Patent metadata

Field	Value
Publication number	US-9645149-B2
Application number	US-201214347073-A
Country	US
Kind code	B2
Filing date	Sep 28, 2012
Priority date	Sep 30, 2011
Publication date	May 9, 2017
Grant date	May 9, 2017

Abstract

This disclosure provides a system for detecting rare cells. The system includes a substrate, an extension coupled to the substrate and extending outwardly from the substrate, and a functionalized graphene oxide disposed on the extension. This disclosure also provides a method for detecting rare cells using the system of this disclosure. The method includes the steps of providing the system and introducing a sample of bodily fluid to the system such that the sample interacts with the functionalized graphene oxide.

First claim

What is claimed is:

1. A system for detecting rare cells, said system comprising:
A. a substrate;
B. an extension coupled to said substrate and extending outwardly from said substrate; and
C. a functionalized graphene oxide disposed on said extension, wherein said functionalized graphene oxide includes a functionalization comprising a binding agent for interaction with the rare cells to immobilize the rare cells on said extension when the rare cells come into contact with said functionalized graphene oxide;
wherein said extension has a height of from 1 to 5 nanometers.

2. The system of claim 1 wherein said functionalized graphene oxide has a thickness of from 1 to 5 nanometers.

3. The system of claim 2 wherein said extension is gold.

4. The system of claim 1 wherein said binding agent comprises a linker, with said linker being phospholipid-polyethylene-glyco-amine.

5. The system of claim 1 wherein said binding agent comprises a linker, with said linker comprising the reaction product of phospholipid-polyethylene-glyco-amine and N-γ-maleimidobutyryloxy succinimide ester.

6. The system of claim 5 wherein said reaction product is bonded to a protein.

7. The system of claim 6 wherein said protein is a deglycosylated form of avidin.

8. The system of claim 6 wherein said protein is bonded to an antibody for interaction with the rare cells.

9. The system of claim 8 wherein said antibody is EpCAM.

10. The system of claim 1 that has a rare cell capture efficiency of at least 75 percent determined as the percentage of a total number of rare cells introduced into the system captured on said functionalized graphene oxide.

11. The system of claim 1 , wherein said binding agent comprises the reaction product of phospholipid-polyethylene-glyco-amine and N-γ-maleimidobutyryloxy succinimide ester, wherein said reaction product is further bonded to a protein, and wherein said protein is further bonded to an antibody for interaction with the rare cells.

12. The system of claim 1 that is further defined as a microfluidic device.

13. A microfluidic device comprising:
A. a silicon substrate;
B. an extension that is a metal, that is coupled to said substrate, and that extends outwardly from said substrate; and
C. a functionalized graphene oxide disposed on said extension, wherein the functionalized graphene oxide includes a functionalization comprising a binding agent for interaction with the rare cells to immobilize the rare cells on said extension when the rare cells come into contact with said functionalized graphene oxide;
wherein said extension has a height of from 1 to 5 nanometers.

14. The microfluidic device of claim 13 wherein said metal is gold, said binding agent comprises the reaction product of phospholipid-polyethylene-glyco-amine and N-γ-maleimidobutyryloxy succinimide ester, said reaction product is further bonded to a protein, and said protein is further bonded to an antibody for interaction with the rare cells.

15. A method for capturing rare cells using a system comprising a substrate, an extension coupled to the substrate and extending outwardly from the substrate with the extension having a height of from 1 to 5 nanometers, and a functionalized graphene oxide disposed on the extension with the functionalized graphene oxide including a functionalization comprising a binding agent, said method comprising the steps of:
I. providing the system; and
II. introducing a sample of bodily fluid to the system such that the sample interacts with the functionalized graphene oxide to immobilize the rare cells on the extension when the rare cells come into contact with the functionalized graphene oxide.

16. The method of claim 15 wherein the functionalized graphene oxide has a thickness of from 1 to 5 nanometers.

17. The method of claim 15 wherein the extension is gold.

18. The method of claim 17 wherein the binding agent comprises the reaction product of phospholipid-polyethylene-glyco-amine and N-γ-maleimidobutyryloxy succinimide ester, wherein the reaction product is further bonded to a protein, and wherein the protein is further bonded to an antibody for interaction with the rare cells.

Assignees

• Univ Michigan Regents

Inventors

• Nagrath Sunitha
• Yoon Hyeun Joong

Classifications

• G01N33/5759

  involving compounds localised on the membrane of tumour or cancer cells · CPC title

• B01L3/5027

  by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip · CPC title

• G01N33/575 Primary

  for cancer · CPC title

• G01N33/54353 Primary

  with ligand attached to the carrier via a chemical coupling agent (coatings G01N33/54393) · CPC title

• G01N33/574 Primary

  Physics · mapped topic