Fluidic flow cytometry devices and methods

Patent metadata

Field	Value
Publication number	US-9645010-B2
Application number	US-201213593488-A
Country	US
Kind code	B2
Filing date	Aug 23, 2012
Priority date	Mar 10, 2009
Publication date	May 9, 2017
Grant date	May 9, 2017

Abstract

Devices, systems and methods facilitate analyzing, identifying and sorting particles in fluids, including cytometry devices and techniques. The described techniques can be used in a variety of applications such as in chemical or biological testing and diagnostic measurements. One exemplary flow cytometry device includes a channel that is capable of conducting a fluid containing at least one particle and also capable of allowing light be transmitted to and from the channel. The flow cytometry device also includes a lens that is positioned between the channel and a color filter. The lens directs at least a portion of light transmitted from the channel to the color filter. The color filter includes a plurality of zones, where each zone is adapted to allow transmission of only a particular spectral range of light. The flow cytometry device further includes a detector configured to receive the light that is transmitted through the color filter.

First claim

What is claimed is:

1. A flow cytometry device, comprising:
a channel capable of conducting a fluid containing at least one particle, the channel further capable of allowing light be transmitted to and from the channel;
a lens positioned between the channel and a single color filter and adapted to direct at least a portion of light transmitted from the channel to the single color filter, the single color filter having a continuously graded transmission spectrum from a first wavelength to a second wavelength without an abrupt boundary, the single color filter positioned at a stationary location with respect to the lens, the lens positioned to direct the light emanating from a corresponding range of positions of the at least one particle to impinge on only a portion of the single color filter as the at least one particle moves along the channel so that the light emanating from the particle at a first range of positions of the particle in the channel is directed by the lens to impinge on only a first portion of the single color filter, and as the particle transitions from the first range of positions to a second range of positions within the channel, the light emanating from the particle is directed to transition from falling on only the first portion to fall only on a second portion of the single color filter that allows transmission of light in a different spectral range of the transmission spectrum compared to the first portion of the single color filter; and
a detector configured to receive the light that is transmitted through the single color filter to produce detection signals in digital form; and
a processor implemented in electronic circuitry to receive the detection signals and configured to process the digital data of the detection signals to determine at least one characteristic of interest of the at least one particle, wherein the processor is further configured to filter the detection signals into a plurality of spectral bands within the transmission spectra of the single color filter, wherein a number of filtered spectral bands and spectral bandwidth of each filtered spectral band is changeable by the processor.

2. The flow cytometry device of claim 1 , further comprising:
at least one light source positioned to direct light to the mirror;
one or more mirrors positioned to reflect the light from the light source(s) into the channel, wherein the one or more mirrors are substantially transparent to the light transmitted by the channel; and
an optical filter positioned between the lens and the detector and adapted to substantially block the light from the light source and to substantially transmit the light transmitted from the channel into the single color filter.

3. The flow cytometry device of claim 2 , wherein at least one of the one or more mirrors is positioned between one of:
the lens and the detector, and
the lens and the channel.

4. The flow cytometry device of claim 1 , further comprising an additional lens positioned between the color filter and the detector.

5. The flow cytometry device of claim 1 , wherein each portion of the single color filter allows transmission of a spectral range of light that is non-overlapping with spectral ranges allowed to be transmitted by all other portions.

6. The flow cytometry device of claim 1 , wherein at least one portion of the single color filter is adapted to allow transmission of a spectral range of light that is overlapping with transmission spectral range of at least one other portion.

7. An imaging device, comprising:
a stage configured to move in one or more dimensions and configured to receive an object for imaging, wherein at least a portion of the object is capable of transmitting light; and
a lens positioned between the stage and an image sensor and adapted to direct the light emitted by the at least a portion of the object to the image sensor,
wherein the image sensor comprises a plurality of light sensing elements arranged as a plurality of sensing element subsets, each sensing element subset being responsive to a particular spectral range of light incident thereupon,
a single continuously graded color filter positioned between the lens and the image sensor, the single continuously graded color filter having a continuously graded transmission spectrum from a first wavelength to a second wavelength without an abrupt boundary, and
wherein the image sensor is aligned with the lens and the object so as to receive, at a first subset of the sensing elements, a first spectral range of light emitted by the object when the stage positions the object at a first position, and to receive a second spectral range of light emitted by the object at a second subset of sensing elements when the stage positions the object at a second position,
the imaging device further comprising a processor implemented in electronic circuitry to receive detection signals produced by the image sensor and configured to process the detection signals to determine at least one characteristic of interest of the object, wherein the processor is further configured to filter the detection signals into a plurality of spectral bands within the transmission spectra of the single continuously graded color filter, wherein a number of filtered spectral bands and spectral bandwidth of each filtered spectral band is changeable by the processor.

8. The imaging device of claim 7 , wherein each portion of the color filter is adapted to allow transmission of only a particular spectral range of light.

9. A method for identifying one or more particles by flow cytometry, comprising:
flowing one or more particles through a channel of a flow cytometry device;
causing the one or more particles to emit light;
receiving light emitted by the one or more particles after transmission through a lens that directs the light to a single color filter, wherein the single color filter comprises a plurality of contiguously graded transmission spectrum from a first wavelength to a second wavelength without an abrupt boundary, and wherein the single color filter is positioned at a stationary location with respect to the lens, the lens positioned to direct the light emanating from a corresponding range of positions of each particle to impinge on only a portion of the single color filter as each particle moves along the channel so that the light emanating from the particle at a first range of positions of the particle in the channel is directed by the lens to impinge on only a first portion of the single color filter, and as the particle transitions from the first range of positions to a second range of positions within the channel, the light emanating from the particle is directed to transition from falling on only the first portion to fall only on a second portion of the single color filter that allows transmission of light in a different spectral range of the transmission spectrum compared to the first portion of the single color filter;
producing digital data associated with the received light; and
processing the digital data to identify the one or more particles, wherein processing the digital data comprises selecting a plurality of dynamically selectable spectral bands, and filtering the digital data into the plurality of spectral bands within the transmission spectra of the single color filter, wherein a number of selected spectral bands and a spectral bandwidth of each selected spectral band is changeable within the spectra of the single color filter.

10. The method of claim 9 , further comprising determining velocity of the one or more particles flowing through the channel.

11. The method of claim 10 , further comprising directing at least one of the one or more particles through a cha

Assignees

• Lo Yu-Hwa
• Cho Sung Hwan
• Morachis Jose
• Alaynick Will
• Chuang Kendall
• Kim Nam
• Univ California
• Nanocellect Biomedical Inc

Inventors

• Lo Yu-Hwa
• Cho Sung Hwan
• Morachis Jose
• Alaynick Will
• Chuang Kendall
• Kim Nam

Classifications

• G01N15/1434

  Optical arrangements · CPC title

• G01J3/28

  Investigating the spectrum (using colour filters G01J3/51) · CPC title

• G01N2015/144

  Imaging characterised by its optical setup · CPC title

• G01P5/26

  by measuring the direct influence of the streaming fluid on the properties of a detecting optical wave · CPC title

• G01J3/51

  using colour filters · CPC title