Temperature responsive adsorbent having a strong cation exchange group and method for producing the same

Patent metadata

Field	Value
Publication number	US-9643173-B2
Application number	US-201113994255-A
Country	US
Kind code	B2
Filing date	Dec 19, 2011
Priority date	Dec 17, 2010
Publication date	May 9, 2017
Grant date	May 9, 2017

Abstract

Provided is a temperature responsive adsorbent prepared by immobilizing a copolymer containing at least N-isopropylacrylamide to a base material surface. The copolymer has at least a strong cation exchange group. In addition, the copolymer contains the strong cation exchange group in an amount of 0.01 to 5 mol % relative to N-isopropylacrylamide in terms of monomer.

First claim

The invention claimed is:

1. A temperature responsive adsorbent capable of separating a physiologically active substance comprising a copolymer containing at least N-isopropylacrylamide immobilized to a support material surface, wherein at least a portion of monomer units of the copolymer has sulfonic acid group, and the copolymer contains the sulfonic acid group in an amount of 0.01 to 5 mol % relative to the N-isopropylacrylamide in terms of monomer,
wherein at least a portion of the monomer units of the copolymer having the sulfonic acid group is an acrylic acid derivative or a methacrylic acid derivative and has a group represented by the following chemical formula (1) or (2):
—CH(—OH)—CH 2 —SO 3 H  (1)

—CH(—SO 3 H)—CH 2 —OH  (2).

2. A method for separating a physiologically active substance comprising adsorbing the physiologically active substance to the adsorbent by use of the adsorbent according to claim 1 and eluting the physiologically active substance by temperature change.

3. The method for separating a physiologically active substance according to claim 2 , wherein the physiologically active substance is an immunoglobulin.

4. A method for producing a temperature responsive adsorbent capable of separating a physiologically active substance, the temperature responsive adsorbent comprising a copolymer containing at least N-isopropylacrylamide immobilized to a support material surface, wherein at least a portion of monomer units of the copolymer has sulfonic acid group, and the copolymer contains the sulfonic acid group in an amount of 0.01 to 5 mol % relative to the N-isopropylacrylamide in terms of monomer,
wherein at least a portion of the monomer units of the copolymer having the sulfonic acid group is an acrylic acid derivative or a methacrylic acid derivative and has a group represented by the following chemical formula (1) or (2):
—CH(—OH)—CH 2 —SO 3 H  (1)

—CH(—SO 3 H)—CH 2 —OH  (2)

wherein the copolymer is a copolymer of at least the N-isopropylacrylamide and a vinyl monomer including a monomer having the sulfonic acid group and/or a precursor monomer for introducing the sulfonic acid group, and
wherein a monomer composition containing the N-isopropylacrylamide and the monomer having the sulfonic acid group and/or the precursor monomer for introducing the sulfonic acid group is polymerized by a surface graft polymerization method to introduce the sulfonic acid group onto the support material surface.

5. The method for producing a temperature responsive adsorbent capable of separating a physiologically active substance according to claim 4 , wherein the copolymer is obtained by copolymerizing a monomer composition containing the N-isopropylacrylamide and the precursor monomer for introducing the sulfonic acid group, and thereafter, performing a reaction for converting the precursor into the sulfonic acid group.

6. The method for producing a temperature responsive adsorbent capable of separating a physiologically active substance according to claim 4 , wherein the precursor monomer for introducing the sulfonic acid group is a vinyl monomer having an epoxy group.

7. The method for producing a temperature responsive adsorbent capable of separating a physiologically active substance according to claim 4 , wherein the copolymer is obtained by copolymerizing a monomer composition containing the N-isopropylacrylamide and the monomer having the sulfonic acid group.

8. The method for producing a temperature responsive adsorbent capable of separating a physiologically active substance according to claim 4 , wherein the copolymer is obtained by polymerizing a monomer composition containing the monomer having the sulfonic acid group and not containing the precursor monomer for introducing the sulfonic acid group.

9. A temperature responsive adsorbent capable of separating a physiologically active substance comprising a copolymer containing at least N-isopropylacrylamide immobilized to a support material surface, wherein at least a portion of monomer units of the copolymer has a sulfonic acid group, and the copolymer contains the sulfonic acid group in an amount of 0.01 to 5 mol % relative to the N-isopropylacrylamide in terms of monomer,
wherein at least a portion of the monomer units of the copolymer having the sulfonic acid group is derived from a vinyl monomer having a sulfonic acid group.

10. The temperature responsive adsorbent according to claim 9 , wherein at least a portion of the monomer units of the copolymer having the sulfonic acid group is represented by the following chemical formula (3):
—CR 1 R 2 —CR 3 (—SO 3 H)—  (3)

where R 1 , R 2 , R 3 are each independently H or Me.

Assignees

• Koguma Ichiro
• Shigematsu Hiroki
• Okuyama Kazuo
• Okano Teruo
• Akiyama Yoshikatsu
• Nagase Kenichi
• Asahi Kasei Medical Co Ltd

Inventors

• Koguma Ichiro
• Shigematsu Hiroki
• Okuyama Kazuo
• Okano Teruo
• Akiyama Yoshikatsu
• Nagase Kenichi

Classifications

• B01J39/19

  obtained otherwise than by reactions only involving unsaturated carbon-to-carbon bonds · CPC title

• C08F293/005

  using free radical "living" or "controlled" polymerisation, e.g. using a complexing agent · CPC title

• B01J20/285

  based on polymers · CPC title

• B01J39/20 Primary

  obtained by reactions only involving unsaturated carbon-to-carbon bonds · CPC title

• C07K1/18

  Ion-exchange chromatography · CPC title