Engineered particles with polarization contrast and alignment control for enhanced imaging

Patent metadata

Field	Value
Publication number	US-9642923-B2
Application number	US-201414188634-A
Country	US
Kind code	B2
Filing date	Feb 24, 2014
Priority date	Feb 24, 2014
Publication date	May 9, 2017
Grant date	May 9, 2017

Abstract

An engineered particle for detecting analytes in an environment includes an electromagnetic receiver that is configured to preferentially receive electromagnetic radiation of a specified polarization relative to the orientation of the electromagnetic receiver. The engineered particle additionally includes an energy emitter coupled to the electromagnetic receiver such that a portion of electromagnetic energy received by the electromagnetic receiver is transferred to and emitted by the energy emitter. The engineered particles are functionalized to selectively interact with an analyte. The engineered particle can additionally be configured to align with a directed energy field in the environment. The selective reception of electromagnetic radiation of a specified polarization and/or alignment with a directed energy field can enable orientation tracking of individual engineered particles, imaging in high-noise environments, or other applications. A method for detecting properties of the analyte of interest by interacting with the engineered particle is also provided.

First claim

What is claimed is:

1. A contrast agent comprising a plurality of engineered particles, wherein each engineered particle of the contrast agent comprises:
a bioreceptor, wherein the bioreceptor selectively interacts with an analyte;
at least one conductive nanorod, wherein the at least one conductive nanorod absorbs polarized electromagnetic radiation that is aligned with an axis of the at least one conductive nanorod more than polarized electromagnetic radiation that is not aligned with the axis of the at least one conductive nanorod, wherein the conductive nanorod transduces a portion of electromagnetic energy received by the at least one conductive nanorod into a surface plasmon resonance, wherein the surface plasmon resonance generates a time-varying electric field around the conductive nanorod; and
at least one light emitter, wherein the at least one light emitter is located within the time-varying electric field that is generated around the conductive nanorod when the conductive nanorod transduces a portion of received electromagnetic energy into a surface plasmon resonance, and wherein the at least one light emitter transduces a time-varying electromagnetic field to which it is exposed into emitted light energy.

2. The contrast agent of claim 1 , wherein the conductive nanorod is attached to a first region of the bioreceptor and the light emitter is attached to a second region of the bioreceptor, and wherein the bioreceptor undergoes a conformational change in response to interacting with the analyte such that a distance between the first region of the bioreceptor and the second region of the bioreceptor is related to whether the bioreceptor is interacting with the analyte.

3. The contrast agent of claim 1 , wherein the at least one light emitter comprises a fluorophore.

4. The contrast agent of claim 1 , wherein the at least one light emitter comprises a quantum dot.

5. The contrast agent of claim 1 , wherein the conductive nanorod comprises superparamagnetic iron oxide coated in gold.

6. The contrast agent of claim 1 , wherein each engineered particle of the contrast agent further comprises a particle of superparamagnetic iron oxide, wherein the particle of superparamagnetic iron oxide experiences a torque when exposed to a magnetic field.

7. The contrast agent of claim 1 , wherein the bioreceptor and the light emitter are disposed on a surface of the conductive nanorod.

8. The contrast agent of claim 1 , wherein the bioreceptor comprises a protein, and wherein the light emitter comprises a fluorescent moiety of the protein.

9. The contrast agent of claim 8 , wherein the bioreceptor comprises a single recombinant protein.

10. The contrast agent of claim 1 , wherein the light emitter comprises a Raman dye.

11. The contrast agent of claim 1 , wherein the bioreceptor comprises an antibody.

12. The contrast agent of claim 1 , wherein the bioreceptor comprises an aptamer.

Assignees

• Verily Life Sciences Llc

Inventors

• Homyk Andrew
• Acosta Victor Marcel
• Bajaj Vikram Singh

Classifications

• A61B5/1468

  using chemical or electrochemical methods, e.g. by polarographic means · CPC title

• A61K49/0093

  Nanoparticle, nanocapsule, nanobubble, nanosphere, nanobead, i.e. having a size or diameter smaller than 1 micrometer, e.g. polymeric nanoparticle · CPC title

• A61B5/0095

  by applying light and detecting acoustic waves, i.e. photoacoustic measurements · CPC title

• A61B5/0051

  by applying vibrations · CPC title

• A61B5/0022

  Monitoring a patient using a global network, e.g. telephone networks, internet · CPC title