Polypeptides having catalase activity and polynucleotides encoding same

Patent metadata

Field	Value
Publication number	US-9637725-B2
Application number	US-201214366127-A
Country	US
Kind code	B2
Filing date	Dec 19, 2012
Priority date	Dec 19, 2011
Publication date	May 2, 2017
Grant date	May 2, 2017

Abstract

Provided are isolated polypeptides having catalase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

First claim

What is claimed is:

1. A process for degrading or hydrolyzing a cellulosic material, comprising: contacting the cellulosic material with a cellulolytic enzyme composition and an isolated polypeptide to thereby degrade or hydrolyze the cellulosic material, wherein said isolated polypeptide has catalase activity and is selected from the group consisting of:
a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the sequence of amino acids 20 to 741 of SEQ ID NO: 8, and
b) a fragment of the sequence of amino acids 20 to 741 of SEQ ID NO: 8, wherein the fragment has catalase activity.

2. The process of claim 1 , wherein said isolated polypeptide comprises an amino acid sequence having at least 97% sequence identity to the sequence of amino acids 20 to 741 of SEQ ID NO: 8.

3. The process of claim 1 , wherein said isolated polypeptide comprises an amino acid sequence having at least 98% sequence identity to the sequence of amino acids 20 to 741 of SEQ ID NO: 8.

4. The process of claim 1 , wherein said isolated polypeptide comprises an amino acid sequence having at least 99% sequence identity to the sequence of amino acids 20 to 741 of SEQ ID NO: 8.

5. The process of claim 1 , wherein said isolated polypeptide comprises the sequence of amino acids 20 to 741 of SEQ ID NO: 8.

6. The process of claim 1 , wherein said isolated polypeptide is encoded by a polynucleotide that hybridizes under high stringency conditions with the full-length complement of the nucleic acid sequence of nucleotides 58 to 2476 of SEQ ID NO: 7, wherein the high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide for 12 to 24 hours, followed by washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.

7. The process of claim 1 , wherein said isolated polypeptide is encoded by a polynucleotide comprising a nucleic acid sequence having at least 95% sequence identity to the nucleic acid sequence of nucleotides 58 to 2476 of SEQ ID NO: 7.

8. The process of claim 1 , wherein said isolated polypeptide is encoded by a polynucleotide comprising a nucleic acid sequence having at least 97% sequence identity to the nucleic acid sequence of nucleotides 58 to 2476 of SEQ ID NO: 7.

9. The process of claim 1 , wherein said isolated polypeptide is encoded by a polynucleotide comprising a nucleic acid sequence having at least 99% sequence identity to the nucleic acid sequence of nucleotides 58 to 2476 of SEQ ID NO: 7.

10. The process of claim 1 , wherein said isolated polypeptide is a variant of the sequence of amino acids 20 to 741 of SEQ ID NO: 8, wherein the variant is modified by a substitution, deletion, and/or insertion at one or more positions in the amino acid sequence of 20 to 741 of SEQ ID NO: 8, and wherein said isolated polypeptide comprises an amino acid sequence having at least 95% sequence identity to the sequence of amino acids 20 to 741 of SEQ ID NO: 8 and has catalase activity.

11. A process for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material with a cellulolytic enzyme composition and an isolated polypeptide to produce a saccharified cellulosic material, wherein the isolated polypeptide has catalase activity and is selected from the group consisting of:
i) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the sequence of amino acids 20 to 741 of SEQ ID NO: 8, and
ii) a fragment of the sequence of amino acids 20 to 741 of SEQ ID NO: 8, wherein the fragment has catalase activity;

(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and optionally
(c) recovering the fermentation product from the fermentation.

12. The process of claim 11 , wherein said isolated polypeptide comprises an amino acid sequence having at least 97% sequence identity to the sequence of amino acids 20 to 741 of SEQ ID NO: 8.

13. The process of claim 11 , wherein said isolated polypeptide comprises an amino acid sequence having at least 98% sequence identity to the sequence of amino acids 20 to 741 of SEQ ID NO: 8.

14. The process of claim 11 , wherein said isolated polypeptide comprises an amino acid sequence having at least 99% sequence identity to the sequence of amino acids 20 to 741 of SEQ ID NO: 8.

15. The process of claim 11 , wherein said isolated polypeptide comprises the sequence of amino acids 20 to 741 of SEQ ID NO: 8.

16. The process of claim 11 , wherein said isolated polypeptide is encoded by a polynucleotide that hybridizes under high stringency conditions with the full-length complement of the nucleic acid sequence of nucleotides 58 to 2476 of SEQ ID NO: 7, wherein the high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide for 12 to 24 hours, followed by washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.

17. The process of claim 11 , wherein said isolated polypeptide is encoded by a polynucleotide comprising a nucleic acid sequence having at least 95% sequence identity to the nucleic acid sequence of nucleotides 58 to 2476 of SEQ ID NO: 7.

18. The process of claim 11 , wherein said isolated polypeptide is encoded by a polynucleotide comprising a nucleic acid sequence having at least 97% sequence identity to the nucleic acid sequence of nucleotides 58 to 2476 of SEQ ID NO: 7.

19. The process of claim 11 , wherein said isolated polypeptide is encoded by a polynucleotide comprising a nucleic acid sequence having at least 99% sequence identity to the nucleic acid sequence of nucleotides 58 to 2476 of SEQ ID NO: 7.

20. The process of claim 11 , wherein said isolated polypeptide is a variant of the sequence of amino acids 20 to 741 of SEQ ID NO: 8, wherein the variant is modified by a substitution, deletion, and/or insertion at one or more positions in the amino act sequence of 20 to 741 of SEQ ID NO: 8, and wherein said isolated polypeptide comprises an amino acid sequence having at least 95% sequence identity to the sequence of amino acids 20 to 741 of SEQ ID NO: 8 and has catalase activity.

Assignees

• Novozymes Inc

Inventors

• Liu Ye
• Duan Junxin
• Zhang Yu
• Tang Lan

Classifications

• C12N9/0065 Primary

  acting on hydrogen peroxide as acceptor (1.11) · CPC title

• C12P2201/00

  Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis · CPC title

• C12Y111/01006

  Catalase (1.11.1.6) · CPC title

• C12P2203/00

  Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source · CPC title

• C12P19/02 Primary

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title