Method of producing partially purified extracellular metabolite products from bacillus coagulans and biological applications thereof

Patent metadata

Field	Value
Publication number	US-9635862-B2
Application number	US-201615214699-A
Country	US
Kind code	B2
Filing date	Jul 20, 2016
Priority date	Dec 24, 2013
Publication date	May 2, 2017
Grant date	May 2, 2017

Abstract

Process of inhibiting microbial biofilm formation using extracellular metabolite composition from Bacillus coagulans MTCC 5856 and composition comprising from about 61% w/w of thymol, about 38% w/w of monolaurin and about 1% w/w of magnolol obtained from supercritical fluid extracts of Magnolia officinalis is described.

First claim

We claim:

1. A process of inhibiting microbial biofilm formation, said process comprising a step of bringing into contact biofilm producing microbial cells and a preparation consisting of partially purified extracellular metabolites of probiotic bacterial strain Bacillus coagulans deposited under accession number MTCC 5856, prepared by partially purifying a culture supernatant of said Bacillus coagulans strain, alone or combined with a composition comprising from about 61% w/w of thymol, about 38% w/w of monolaurin, and about 1% w/w of magnolol obtained from supercritical fluid extracts of Magnolia officinalis , thereby inhibiting said biofilm formation.

2. A process of preventing pH drop and further accumulation of biofilm of Streptococcus mutans in presence of sucrose, said process comprising a step of bringing into contact biofilm/plaque producing Streptococcus mutans and a preparation consisting essentially of partially purified extracellular metabolites of probiotic bacterial strain Bacillus coagulans deposited under accession number MTCC 5856, prepared by partially purifying a culture supernatant of said Bacillus coagulans strain, alone or combined with a composition comprising from about 61% w/w of thymol, about 38% w/w of monolaurin, and about 1% w/w of magnolol obtained from supercritical fluid extracts of Magnolia officinalis , thereby preventing pH drop and biofilm accumulation.

3. A process of preventing pH drop and further accumulation of biofilm of Streptococcus mutans in presence of sucrose, said process comprising step of bringing into contact biofilm/plaque producing Streptococcus mutans and a preparation consisting essentially of partially purified extracellular metabolite preparation from probiotic bacterial strain Bacillus coagulans MTCC 5856 alone or said preparation combined with a composition comprising from about 61% w/w of thymol, about 38% w/w of monolaurin and about 1% w/w of magnolol obtained from supercritical fluid extracts of Magnolia officinalis , to prevent such drop in pH and biofilm accumulation.

Assignees

• Majeed Muhammed
• Nagabhushanam Kalyanam
• Arumugam Sivakumar
• Ali Furqan
• Sami Labs Ltd

Inventors

• Majeed Muhammed
• Nagabhushanam Kalyanam
• Arumugam Sivakumar
• Ali Furqan

Classifications

• A01N63/10

  Animals; Substances produced thereby or obtained therefrom · CPC title

• C12N1/20 Primary

  Bacteria; Culture media therefor · CPC title

• A01N37/12

  containing the group [IMAGE cpc-sch-A01N-0932.gif], wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof · CPC title

• A01N31/08

  Oxygen or sulfur directly attached to an aromatic ring system · CPC title

• A01N65/08

  Magnoliopsida [dicotyledons] · CPC title