Detection of antigens

Patent metadata

Field	Value
Publication number	US-9632078-B2
Application number	US-201013383167-A
Country	US
Kind code	B2
Filing date	Jul 6, 2010
Priority date	Jul 9, 2009
Publication date	Apr 25, 2017
Grant date	Apr 25, 2017

Abstract

The invention discloses a method for detecting at least one antigen, comprising the following steps: providing magnetic beads, which are coated with antibodies specific for at least one antigen to be detected; bringing the magnetic beads in contact with a washing buffer that comprises at least 8% BSA and incubating the mixture with a sample; isolating the magnetic beads by means of magnetic separator; and detecting the antigens bound to the magnetic beads by the antibodies. Washing buffers, primers, kits and devices that can be used for said methods are also disclosed.

First claim

The invention claimed is:

1. A method for the detection of at least one antigen in a sample comprising the steps of:
(a) coating magnetic beads with at least one antibody specific for the at least one antigen;
(b) washing the coated beads with a first washing solution comprising a buffer and about 8% to 12% bovine serum albumin (BSA);
(c) incubating the washed beads with the sample to form a mixture;
(d) washing the mixture with a second washing solution comprising a buffer;
(e) isolating the beads using a magnetic separator; and
(f) detecting the at least one antigen in the sample.

2. The method of claim 1 wherein the first washing solution further comprises a detergent.

3. The method of claim 1 wherein the second washing solution further comprises about 8% to 12% BSA.

4. The method of claim 1 wherein the first washing solution and the second washing solution are the same.

5. The method of claim 1 wherein the washing step (b) is performed by incubating the coated beads in the first washing solution for at least 30 minutes to 12 hours.

6. The method of claim 1 wherein detecting the at least one antigen is performed using a nucleic acid amplification method.

7. The method of claim 1 wherein detecting the at least one antigen is performed using an enzyme-linked immunosorbent assay (ELISA) assay.

8. The method of claim 1 where the washing step (b) is repeated at least one time prior to the incubation step (c).

9. The method of claim 1 wherein the washing step (d) is repeated at least one time after the incubation step (c).

10. The method of claim 1 wherein the washing step (d) occurs on a magnetic separator.

11. The method of claim 1 where the at least one antigen to be detected is from a microorganism selected from the group consisting of a bacterium, a virus, a fungus, a toxin, LPS, and protozoa.

12. The method of claim 1 where the at least one antibody comprises an antibody specific for a microorganism selected from the group consisting of a bacterium, a virus, a protozoon, a fungus; and an antibody specific for a toxin produced by the microorganism, the bacterium, the virus, the protozoon, and the fungus.

13. The method of claim 11 wherein the microorganism is selected from Escherichia coli , adenovirus, and Legionella spp.

14. The method of claim 12 wherein the microorganism is selected from Escherichia coli , adenovirus, and Legionella spp.

Assignees

• Reidt Ulrich
• Friedberger Alois
• Heller Christoph
• Brahms Hero
• Airbus Defence & Space Gmbh

Inventors

• Reidt Ulrich
• Friedberger Alois
• Heller Christoph
• Brahms Hero

Classifications

• G01N33/54333 Primary

  Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction · CPC title