Synthesis of prazole compounds

Patent metadata

Field	Value
Publication number	US-9631181-B2
Application number	US-201615211329-A
Country	US
Kind code	B2
Filing date	Jul 15, 2016
Priority date	Dec 8, 2009
Publication date	Apr 25, 2017
Grant date	Apr 25, 2017

Abstract

The present disclosure relates to non-naturally occurring monooxygenase polypeptides useful for preparing prazole compounds, polynucleotides encoding the polypeptides, and methods of using the polypeptides.

First claim

What is claimed is:

1. A non-naturally occurring polynucleotide sequence encoding a non-naturally occurring polypeptide sequence that is at least 90% identical to SEQ ID NO: 158.

2. The non-naturally occurring polynucleotide sequence encoding the non-naturally occurring monooxygenase of claim 1 , wherein the polypeptide is capable of converting the compound (1) to (S)-5-methoxy-2-((4-methoxy-3,5-dimethylpyridin-2-yl)methylsulfinyl)-1H-benzo[d]imidazole (“compound (2b)”) in enantiomeric excess.

3. The non-naturally occurring polynucleotide sequence of claim 1 , wherein the non-naturally occurring polypeptide sequence encoded by said polynucleotide sequence comprises SEQ ID NO: 158.

4. The non-naturally occurring polynucleotide sequence of claim 1 , wherein said non-naturally occurring polynucleotide sequence is at least 90% identical to SEQ ID NO:157.

5. The non-naturally occurring polynucleotide sequence of claim 1 , wherein said sequence comprises SEQ ID NO:157.

6. An expression vector comprising the non-naturally occurring polynucleotide sequence of claim 1 .

7. The expression vector of claim 4 , wherein said vector further comprises at least one control sequence.

8. A host cell comprising the expression vector of claim 6 .

9. A host cell comprising the expression vector of claim 7 .

10. The host cell of claim 8 , wherein said host cell is E. coli.

11. The host cell of claim 9 , wherein said host cell is E. coli.

12. A method for production of a non-naturally occurring polypeptide comprising culturing the host cell of claim 8 , under conditions suitable for production of said non-naturally occurring polypeptide.

13. A method for production of a non-naturally occurring polypeptide comprising culturing the host cell of claim 9 , under conditions suitable for production of said non-naturally occurring polypeptide.

14. The method of claim 12 , further comprising the step of isolating said produced non-naturally occurring polypeptide.

15. The method of claim 13 , further comprising the step of isolating said produced non-naturally occurring polypeptide.

16. A non-naturally occurring polypeptide sequence that is at least 90% identical to SEQ ID NO:158.

17. The non-naturally occurring polypeptide sequence of claim 16 , wherein said non-naturally occurring polypeptide sequence comprises SEQ ID NO:158.

Assignees

• Codexis Inc

Inventors

• Bong Yong Koy
• Clay Michael D
• Collier Steven J
• Mijts Benjamin
• Vogel Michael
• Zhang Xiyun
• Zhu Jun
• Nazor Jovana
• Smith Derek J
• Song Shiwei

Classifications

• C12P17/182

  Heterocyclic compounds containing nitrogen atoms as the only ring heteroatoms in the condensed system (alloxazine or isoalloxazine, e.g. riboflavine C12P25/00) · CPC title

• C12N9/0073 Primary

  with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13 · CPC title

• Y02P20/52

  using catalysts, e.g. selective catalysts · CPC title

• C12Y114/13022

  Cyclohexanone monooxygenase (1.14.13.22) · CPC title

• C12P17/165

  Heterorings having nitrogen atoms as the only ring heteroatoms · CPC title