Microstructure biomaterials and fabrication methods therefor

Patent metadata

Field	Value
Publication number	US-9631171-B2
Application number	US-201113989024-A
Country	US
Kind code	B2
Filing date	Nov 22, 2011
Priority date	Nov 22, 2010
Publication date	Apr 25, 2017
Grant date	Apr 25, 2017

Abstract

Methods and systems for fabricating a micro-structured biomaterial include printing a three-dimensional structure using polymerizing radiation modulated by a digital micromirror array to project microstructure patterns into a pre-polymer material to form one or more porous scaffold sheets. The microstructure patterns have a unit-cell geometry that exhibits a negative Poisson ratio that is tunable in magnitude.

First claim

The invention claimed is:

1. A method of fabricating a microstructure biomaterial scaffold comprising:
(a) designing two-dimensional graphics models of scaffold layers;
(b) generating virtual photomasks of the scaffold layers using the designed two-dimensional graphics model; and
(c) patterning and fabricating each of the scaffold layers using the generated virtual photomasks, wherein at least one of the scaffold layers is a microstructure hybrid layer with a first portion of the microstructure hybrid layer comprising a unit-cell geometry having a negative Poisson ratio and a second portion of the microstructure hybrid layer comprising a unit-cell geometry having a positive Poisson ratio.

2. The method of claim 1 , wherein the scaffold layers comprise porous layers.

3. The method of claim 2 , wherein the Poisson ratio is tuned by controlling pore geometry of each scaffold layer.

4. The method of claim 2 , wherein each scaffold layer is stacked above or below each other and connected by vertical connecting posts.

5. The method of claim 1 , wherein each scaffold layer is stacked above or below each other and connected by vertical connecting posts.

6. A method for fabricating a microstructure biomaterial scaffold comprising:
disposing a transparent plate above a servo stage to define a gap;
injecting a photo-curable polymer into the gap;
modulating light having a wavelength suitable for curing the photo-curable polymer using a digital micro-mirror array, wherein the digital micro-mirror array is controlled by a plurality of virtual software masks for defining a microstructure pattern;
focusing modulated light onto a plane below the transparent plate to cure the photo-curable polymer within the plane with the microstructure pattern; and
removing uncured polymer to reveal a microstructure hybrid layer having the microstructure pattern, wherein at least a first portion of the microstructure hybrid layer comprises a unit-cell geometry having a negative Poisson ratio, and wherein a second portion of the microstructure hybrid layer comprises a second unit-cell geometry having a positive Poisson ratio.

7. The method of claim 6 , wherein the unit-cell geometry comprises a reentrant honeycomb model.

8. The method of claim 6 , wherein the unit-cell geometry comprises a cut missing rib model.

9. The method of claim 6 , further comprising; after removing uncured polymer:
lowering the servo stage to define a second gap;
repeating the steps of injecting, modulating, focusing, and removing and thereby defining a second microstructure hybrid layer having a second microstructure pattern on top of the microstructure hybrid layer.

10. The method of claim 9 , wherein the second microstructure layer comprises a plurality of vertical posts, and further comprising repeating the steps of injecting, modulating, focusing, and removing to define a third microstructure layer having a third microstructure pattern on top of the vertical posts.

11. The method of claim 10 , further comprising:
injecting a sacrificial material into the second gap before repeating the steps of injecting, modulating, focusing, and removing; and
after the removing step, removing the sacrificial material to reveal a multi-layered microstructure.

12. The method of claim 9 , wherein the step of focusing further comprises translating the servo stage in an x-y direction to cure with the microstructure pattern the photo-curable polymer within one or more adjacent areas, whereby a plurality of areas are stitched together to produce a scaffold.

13. The method of claim 9 , further comprising:
injecting a sacrificial material into the second gap before repeating the steps of injecting, modulating, focusing, and removing; and
after the removing step, removing the sacrificial material to reveal a multi-layered microstructure.

14. The method of claim 6 , wherein the second unit-cell geometry comprises an intact rib model.

15. The method of claim 12 , wherein the scaffold has a Poisson ratio tuned by controlling unit-cell geometry of each area.

16. The method of claim 6 , wherein the unit-cell geometry is a reentrant six-sided honeycomb having four side angles between ribs, and wherein the negative Poisson ratio is tuned by changing one or more of the side angles and lengths of the ribs.

17. The method of claim 6 , wherein the unit-cell geometry is a reentrant honeycomb having four side angles between ribs, and wherein the negative Poisson ratio is tuned by changing a direction of loading relative to an orientation of the unit-cell.

Assignees

• Soman Pranav
• Chen Shaochen
• Fozdar David
• Univ California

Inventors

• Soman Pranav
• Chen Shaochen
• Fozdar David

Classifications

• A61L27/14 Primary

  Macromolecular materials · CPC title

• A61L2400/12

  Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces · CPC title

• A61L2400/18

  Modification of implant surfaces in order to improve biocompatibility, cell growth, fixation of biomolecules, e.g. plasma treatment · CPC title

• A61L27/40

  Composite materials, i.e. containing one material dispersed in a matrix of the same or different material · CPC title

• C12N5/00 Primary

  Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor; (plant reproduction by tissue culture techniques A01H4/00) · CPC title