Selective removal of a protein from a mixture of proteins using activated carbon by adjusting solution conditions
US-2015376232-A1 · Dec 31, 2015 · US
Immunoglobulin purification
US9631008B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9631008-B2 |
| Application number | US-200913141306-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 18, 2009 |
| Priority date | Dec 22, 2008 |
| Publication date | Apr 25, 2017 |
| Grant date | Apr 25, 2017 |
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Abstract
Official abstract text for this publication.
The current invention reports a method for purifying an immunoglobulin, wherein the method comprises applying an aqueous, buffered solution comprising an immunoglobulin in monomeric, in aggregated, and in fragmented form to an anion exchange chromatography material under conditions whereby the immunoglobulin in monomeric form does not bind to the anion exchange material, and recovering the immunoglobulin in monomeric form in the flow-through from the anion exchange chromatography material, whereby the buffered aqueous solution has a pH value of from 8.0 to 8.5. In one embodiment the anion exchange chromatography material is a membrane anion exchange chromatography material.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method for obtaining an immunoglobulin in monomeric form, wherein the method comprises the following step: applying an aqueous, buffered solution comprising an immunoglobulin in monomeric and in aggregated form and immunoglobulin fragments to a membrane anion exchange chromatography material, wherein the aqueous, buffered solution has a pH value of pH 8.5, whereby the immunoglobulin depleted of immunoglobulin aggregates and immunoglobulin fragments is recovered from the flow-through of the membrane anion exchange chromatography material and thereby an immunoglobulin in monomeric form is obtained. 2. The method according to claim 1 , characterized in that said anion exchange chromatography material is a strong anion exchange chromatography material. 3. The method according to any one of the preceding claims, characterized in that said method comprises prior to step a) an additional protein A chromatography step or a HCIC chromatography step.
Assignees
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Classifications
Stabilisation, fragmentation · CPC title
against receptors for cytokines, lymphokines, interferons · CPC title
against the immunoglobulin superfamily · CPC title
Ion-exchange chromatography · CPC title
- C07K16/065Primary
Purification, fragmentation · CPC title
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- What does patent US9631008B2 cover?
- The current invention reports a method for purifying an immunoglobulin, wherein the method comprises applying an aqueous, buffered solution comprising an immunoglobulin in monomeric, in aggregated, and in fragmented form to an anion exchange chromatography material under conditions whereby the immunoglobulin in monomeric form does not bind to the anion exchange material, and recovering the immu…
- Who is the assignee on this patent?
- Pompiati Marc, Schaubmar Andreas, Hoffmann La Roche
- What technology area does this patent fall under?
- Primary CPC classification C07K16/065. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Apr 25 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).