βsheet tapes ribbons in tissue engineering

Patent metadata

Field	Value
Publication number	US-9630991-B2
Application number	US-201514887592-A
Country	US
Kind code	B2
Filing date	Oct 20, 2015
Priority date	Jul 15, 2002
Publication date	Apr 25, 2017
Grant date	Apr 25, 2017

Abstract

There is described a material comprising tapes, ribbons, fibrils or fibers characterized in that each of the ribbons, fibrils or fibers have an antiparallel arrangement of peptides in a β-sheet tape-like substructure.

First claim

The invention claimed is:

1. A self assembling polypeptide (SAP) that forms ribbons, fibrils or fibres in a β-sheet tape-like substructure at physiological pH and physiological salt concentrations, and wherein the polypeptide comprises P 11-17 (TTRFEWEFETT, SEQ ID NO: 9).

2. A dental product comprising the self-assembling polypeptide of claim 1 .

3. A dental repair or reconstruction or preventative product comprising the self-assembling polypeptide of claim 1 .

4. A tissue engineering scaffold comprising the self-assembling polypeptide of claim 1 .

5. The tissue engineering scaffold according to claim 4 , wherein the scaffold is seeded with cells.

6. A bone repair or reconstruction product comprising the self-assembling polypeptide of claim 1 .

7. The bone repair or reconstruction product according to claim 6 , wherein the product is seeded with cells.

8. A soft tissue or reconstruction product comprising the self-assembling polypeptide of claim 1 .

9. A skin treatment comprising the self-assembling polypeptide of claim 1 .

10. A hair care product comprising the self-assembling polypeptide of claim 1 .

11. A bioresponsive and biocompatible surface comprising the self-assembling polypeptide of claim 1 .

12. The self-assembling polypeptide of claim 1 , comprising continuous filaments, yarns, staple, floc, cord, or fabric.

13. The self-assembling polypeptide of claim 1 , further comprising a polymer.

14. The self-assembling polypeptide of claim 1 , wherein the physiological pH is about 7.5.

15. The self-assembling polypeptide of claim 1 , wherein the physiological salt concentration is about 145 mM NaCl.

16. A composition, comprising:
ribbons, fibrils or fibres, wherein each of the ribbons, fibrils or fibres has an antiparallel arrangement of peptides in a β-sheet tape-like substructure at physiological pH and physiological salt concentrations, wherein the composition comprises peptide P 11-17 (TTRFEWEFETT, SEQ ID NO: 9); and a physiological concentration of salt.

17. The composition of claim 16 , wherein the composition comprises fibrils, and wherein the fibrils are comprised in a network of fibrils interconnected at fibre-like junctions.

18. A dental composition comprising the fibrils of claim 16 .

19. A dental repair or reconstruction or preventative composition comprising the fibrils of claim 16 .

20. The composition of claim 16 , wherein the composition is in the form of a tissue engineering scaffold.

21. The composition according to claim 20 , comprising the scaffold seeded with cells.

22. A bone repair or reconstruction composition comprising the fibrils of claim 16 .

23. A soft tissue or reconstruction composition comprising the fibrils of claim 16 .

24. The composition according to claim 22 that is seeded with cells.

25. A skin treatment comprising the composition of fibrils of claim 16 .

26. A hair care product comprising the composition of fibrils of claim 16 .

27. A part of a bioresponsive and biocompatible surface comprising the composition of fibrils of claim 16 .

28. The composition of claim 16 , wherein the composition comprises continuous filament yarns, staple, floc, cord, or fabric.

29. The composition of claim 16 , further comprising a polymer.

30. The composition of claim 16 , wherein the physiological pH is about 7.5.

31. The composition of claim 16 , wherein the physiological salt concentration is about 145 mM NaCl.

32. A method of forming a tape, comprising contacting the SAP of claim 1 with an ionic aqueous medium, thereby forming a tape.

33. A method of forming a gel, comprising contacting the SAP of claim 1 with an ionic aqueous medium, thereby forming a gel.

34. A method of forming a tape, comprising contacting the composition of claim 16 with an ionic aqueous medium, thereby forming a tape.

35. A method of forming a gel, comprising contacting the composition of claim 16 with an ionic aqueous medium, thereby forming a gel.

Assignees

• Univ Leeds Innovations Ltd

Inventors

• Boden Neville
• Aggeli Amalia
• Ingham Eileen
• Kirkham Jennifer

Classifications

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• A61L27/38

  containing added animal cells (organs or tissue containing native cells A61L27/36) · CPC title

• A61L27/22

  Polypeptides or derivatives thereof {, e.g. degradation products} · CPC title

• A61L27/56

  Porous materials, {e.g. foams or sponges} · CPC title

• A61L31/047

  Other specific proteins or polypeptides not covered by A61L31/044 - A61L31/046 · CPC title