Responsive liquid crystal elastomers for enhanced cell sheet alignment

Patent metadata

Field	Value
Publication number	US-9625752-B1
Application number	US-201414330691-A
Country	US
Kind code	B1
Filing date	Jul 14, 2014
Priority date	Jul 15, 2013
Publication date	Apr 18, 2017
Grant date	Apr 18, 2017

Abstract

Responsive, biocompatible substrates are of interest for directing the maturation and function of cells in vitro during cell culture. This can potentially provide cells and tissues with desirable properties for regenerative therapies. The present disclosure provides a scalable approach to attach, align and dynamically load cells on responsive liquid crystal elastomer (LCE) substrates. Monodomain LCEs exhibit reversible shape changes in response to cyclic stimulus, and when immersed in an aqueous medium on top of, for example, resistive heaters, shape changes are fast, reversible and produce minimal temperature changes in the surroundings.

First claim

What is claimed is:

1. A cell culture substrate comprising:
a shape-responsive layer comprising a liquid crystal mesogen, a crosslinker and a polymer; and
a cell-adhesion layer comprising a material selected from the group consisting of gold, polystyrene, collagen, fibronectin, laminin, and combinations thereof.

2. The substrate of claim 1 wherein the liquid crystal mesogen is 4-methoxyphenyl 4-(3-butenyloxy) benzoate and the polymer is polymethylhydrosiloxane, and wherein the cell-adhesion layer comprises gold and collagen.

3. The substrate of claim 1 wherein the shape-responsive layer further comprises carbon black nanoparticles.

4. The substrate of claim 3 wherein the carbon black nanoparticles are dispersed throughout the shape-responsive layer with an increased concentration of carbon black nanoparticles at an edge of the shape-responsive layer.

5. The substrate of claim 3 wherein the substrate possesses an electrical resistivity of from about 0.1 Ω·m to about 10.0 Ω·m.

6. The substrate of claim 3 wherein the substrate possesses an elastic modulus of from about 3 kN to about 9 kN.

7. The substrate of claim 3 wherein the substrate possesses a thermo-mechanical strain from about 1% to about 27%.

Assignees

• Univ Rice William M

Inventors

• Verduzco Rafael
• Jacot Jeffrey G
• Adetiba Oluwatomiyin
• Agrawal Aditya

Classifications

• C12N2535/10

  Patterned coating · CPC title

• C09K19/3833

  with mesogenic groups in the side chain · CPC title

• C12N2533/12

  Glass · CPC title

• C12N2533/52

  Fibronectin; Laminin · CPC title

• G02F1/133365 Primary

  Cells in which the active layer comprises a liquid crystalline polymer · CPC title