Synthesis of naringenin nanoparticles

Patent metadata

Field	Value
Publication number	US-9622984-B1
Application number	US-201615141781-A
Country	US
Kind code	B1
Filing date	Apr 28, 2016
Priority date	Apr 28, 2016
Publication date	Apr 18, 2017
Grant date	Apr 18, 2017

Abstract

A method of preparing naringenin nanoparticles comprises dissolving naringenin in an organic solvent to form a solution; adding the solution to boiling water under ultrasonic conditions to form a mixture; and stirring the mixture to obtain the naringenin nanoparticles. The organic solvent can be at least one of methanol, ethanol, dichloromethane and chloroform. Ultrasonic conditions can include applying ultrasonic energy at a frequency of 30-60 kHz and a power of 100 watts for about 20-30 minutes to the mixture.

First claim

We claim:

1. A method of synthesizing naringenin nanoparticles, comprising:
dissolving naringenin in an organic solvent to form a solution;
adding the solution to boiling water, wherein the boiling water and the naringenin solution are both irradiated under ultrasonic conditions to form a mixture;
allowing the mixture to cool; and
stirring the mixture at a temperature of about 30° C. to about 50° C. to obtain naringenin nanoparticles.

2. The method of synthesizing naringenin nanoparticles according to claim 1 , wherein the ultrasonic conditions include irradiation of the mixture with ultrasonic waves having a frequency of about 30 to about 60 kHz and with a power of about 100 watts.

3. The method of preparing naringenin nanoparticles according to claim 1 , wherein the organic solvent is selected from the group consisting of at least one of methanol, ethanol, dichloromethane and chloroform.

4. The method of preparing naringenin nanoparticles according to claim 1 , wherein the solution is added by spraying the solution dropwise into the boiling water at a rate of about 0.2 ml/minute.

5. The method of preparing naringenin nanoparticles according to claim 1 , further comprising isolating the naringenin nanoparticles by at least one of centrifugation and filtration.

6. The method of synthesizing naringenin nanoparticles according to claim 1 , wherein the naringenin nanoparticles have a mean diameter in the range of from about 50 nm to about 200 nm.

7. The method of synthesizing naringenin nanoparticles according to claim 1 , wherein the naringenin nanoparticles have spherical, spheroidal, elongated spherical, rod-shaped, and/or faceted shapes.

8. A method of treating oxidative stress in an organism comprising administering an effective amount of naringenin nanoparticles to the organism, wherein the naringenin nanoparticles are synthesized by the method of claim 1 .

9. The method of treating oxidative stress in an organism according to claim 8 , wherein the organism is freshwater fish.

10. The method of treating oxidative stress in an organism according to claim 9 , wherein the freshwater fish is Nile tilapia.

Assignees

• Univ King Saud

Inventors

• Virk Promy
• Al-Ghamdi Nouf Abdallah Mureet
• Awad Manal Ahmed Gasmelseed
• Wagealla Mai Abdelrahman Elobeid
• Hendi Awatif Ahmed
• Al Hassan Lulwah Saleh Mohammed

Classifications

• A61K9/5192 Primary

  Processes · CPC title

• A61K31/353

  3,4-Dihydrobenzopyrans, e.g. chroman, catechin · CPC title

• A23K20/111

  Aromatic compounds · CPC title

• A23K50/80

  for aquatic animals, e.g. fish, crustaceans or molluscs · CPC title

• A61K9/14

  Particulate form, e.g. powders, {Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles (microspheres A61K9/16; microcapsules A61K9/50; nanocapsules, nanoparticles of the matrix type A61K9/51)} · CPC title