Integrated heater and magnetic separator

Patent metadata

Field	Value
Publication number	US-9618139-B2
Application number	US-17858608-A
Country	US
Kind code	B2
Filing date	Jul 23, 2008
Priority date	Jul 13, 2007
Publication date	Apr 11, 2017
Grant date	Apr 11, 2017

Abstract

An apparatus for providing thermal and magnetic energy to a receptacle containing a reaction mixture and a magnetic retention member. The apparatus can also control heating of a reaction mixture, and bring about a separation of magnetic particles from the reaction mixture. The reaction mixture typically comprises polynucleotides from a biological sample that are being brought into a PCR-ready form.

First claim

What is claimed is:

1. A system for extraction of nucleic acids comprising an integrated separator and heater and a rack removably received in the system, the system comprising:
a heater assembly, wherein the heater assembly comprises a plurality of independently controllable heater units aligned collinearly along a horizontal first axis, each of which is configured to accept and to heat a single process tube of a plurality of process tubes placed in the heater assembly;
one or more magnets affixed to a supporting member, wherein all of the one or more magnets in the system are disposed on a front side of the plurality of independently controllable heater units and aligned along a second axis parallel to the first axis;
a rack removably received in the system, at least a portion of the rack removably received on a back side of the plurality of independently controllable heater units opposite the one or more magnets on the front side;
a plurality of holders, each holder comprising a single process tube of the plurality of process tubes, the plurality of holders removably received in the rack such that a portion of each process tube is received in a single heater unit of the plurality of independently controllable heater units as the rack is inserted into the system;
a motorized mechanism configured to move the supporting member in such a manner that all of the one or more magnets move uniformly backwards and forwards together in a vertical direction perpendicular to the second axis, and during at least a portion of the motion all of the one or more magnets simultaneously maintain proximity to an exterior side of each of the process tubes in the heater assembly sufficient to move magnetic particles contained in the process tubes against a wall of the process tubes; and
control circuitry to control the motorized mechanism and to control heating of the heater units.

2. The integrated heater and separator of claim 1 , wherein both the heating of the plurality of process tubes and the separation of magnetic particles within the process tubes occur when the process tubes are situated in the heater units, and without requiring the process tubes to be removed or repositioned after the heating.

3. The integrated magnetic separator and heater of claim 2 , wherein each of the heater units comprises a power resistor.

4. The integrated heater and separator of claim 1 , wherein each heater unit comprises a heat block having an internal cavity configured to partially surround a lower portion of one of the plurality of process tubes on at least two sides.

5. The integrated heater and separator of claim 4 , wherein each heater unit is comprised of an open face on at least one side and each of the one or more magnets face an open face of the heater unit when the magnet is in close proximity to the process tube.

6. The integrated heater and separator of claim 1 , wherein each heater unit comprises a heat block, wherein the heat block is made of a material selected from: aluminum; silver; gold; copper; and alloys thereof.

7. The integrated heater and separator of claim 1 , configured to heat a 1 ml biological sample in one of the process tubes from room temperature to 65° C. in less than 3 minutes.

8. The integrated heater and separator of claim 1 , wherein the heater assembly further comprises one or more temperature sensors configured to sense and record the respective temperatures of each of the heat blocks.

9. The integrated heater and separator of claim 1 , wherein the motorized mechanism comprises a shaft to which the supporting member is attached, and a motor configured to move the supporting member along the shaft.

10. The integrated heater and separator of claim 9 , wherein the supporting member is configured to move between a first position, situated away from the plurality of process tubes, and a second position situated in close proximity to the plurality of process tubes, and is further configured to move at an amplitude about the second position where the amplitude is smaller than a distance between the first position and the second position as measured along the shaft.

11. The integrated separator and heater of claim 1 , wherein there are 12 heater units.

12. The integrated separator and heater of claim 4 , wherein the heat block comprises two sides perpendicular to each other.

13. The integrated separator and heater of claim 4 , wherein the heat block surrounds the lower portion of the process tube on two sides.

14. The integrated separator and heater of claim 4 , wherein the heat block surrounds the lower portion of the process tube on three sides.

15. The integrated separator and heater of claim 1 , wherein each of the process tubes comes within 1 mm to at most only one magnet.

16. A diagnostic apparatus comprising the integrated separator and heater of claim 1 .

Assignees

• Handique Kalyan
• Handylab Inc

Inventors

• Handique Kalyan

Classifications

• B01L3/5027

  by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip · CPC title

• B01L2200/16

  Reagents, handling or storing thereof · CPC title

• F16K99/0061

  actuated by an expanding gas or liquid volume · CPC title

• G01N35/0098

  involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation (magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C) · CPC title

• B01L2300/1861

  using radiation · CPC title