Plant seeds with altered storage compound levels, related constructs and methods involving genes encoding oxidoreductase motif polypeptides

Patent metadata

Field	Value
Publication number	US-9617556-B2
Application number	US-201314082567-A
Country	US
Kind code	B2
Filing date	Nov 18, 2013
Priority date	Mar 3, 2010
Publication date	Apr 11, 2017
Grant date	Apr 11, 2017

Abstract

This invention is in the field of plant molecular biology. More specifically, this invention pertains to isolated nucleic acid fragments encoding ORM proteins in plants and seeds and the use of such fragments to modulate expression of a gene encoding ORM protein activity in a transformed host cell.

First claim

What is claimed is:

1. A method for producing a dicot plant, the method comprising:
a) obtaining a modified plant cell comprising a polynucleotide operably linked to at least one regulatory sequence, wherein the polynucleotide encodes a polypeptide having an amino acid sequence of at least 95% sequence identity when compared to SEQ ID NO: 36 or 38; and
(b) regenerating a dicot plant from the plant cell of (a);
wherein seed obtained from said plant exhibits an altered oil, protein, starch or soluble carbohydrate content when compared to a seed from a control plant not comprising said polynucleotide.

2. Seed obtained by the method of claim 1 , wherein the seed is soybean seed, comprises the polynucleotide and exhibits an altered oil or protein content when compared to a seed from a control plant not comprising said polynucleotide.

3. The seed of claim 2 , wherein the seed exhibits an increase in oil content when compared to a seed from a control plant not comprising said polynucleotide.

4. The method of claim 1 , wherein the seed obtained from the plant regenerated in step (b) has increased oil content compared with the oil content of seed from the control plant.

5. The method of claim 4 , wherein the plant regenerated in step (b) is a soybean plant.

6. A method for producing dicot seeds, the method comprising:
(a) obtaining a modified plant cell comprising a polynucleotide operably linked to at least one regulatory sequence, wherein the polynucleotide encodes a polypeptide having an amino acid sequence of at least 95% sequence identity to SEQ ID NO: 36 or 38, and is expressed such that expression of endogenous oxidoreductase motif (ORM) protein in the plant cell is reduced;
(b) regenerating a dicot plant from the plant cell of (a); and
(c) selecting a plant that produces a seed having an altered oil, protein, starch or soluble carbohydrate content, as compared to a control seed obtained from a control plant not comprising said polynucleotide.

7. The method of claim 6 , wherein the seed has increased oil content compared with the oil content of the control seed.

8. The method of claim 7 , wherein the seed is a soybean seed.

9. Seed obtained by the method of claim 6 , wherein the seed is soybean seed, comprises the modified polynucleotide and exhibits an altered oil or protein content when compared to a seed from a control plant not comprising said polynucleotide.

10. The seed of claim 9 , wherein the seed exhibits an increase in oil content when compared to a seed from a control plant not comprising said polynucleotide.

11. The method of claim 6 , wherein said seed has an increased oil content of at least 2% when compared to the oil content of the control seed.

12. A transformed dicot plant comprising
a polynucleotide stably transformed in the genome of the plant, wherein the polynucleotide encodes a polypeptide having an amino acid sequence of at least 95% sequence identity when compared to SEQ ID NO: 36 or 38; and wherein seed obtained from said transformed dicot plant has altered oxidoreductase motif (ORM) protein activity and altered oil, protein, starch or soluble carbohydrate content when compared to a seed from a control plant not comprising said polynucleotide.

13. The transformed plant of claim 12 , wherein the seed obtained from the transformed plant has increased oil content compared with the oil content of the control seed.

14. The transformed plant of claim 13 , wherein the transformed plant is a soybean plant.

15. A transformed dicot seed comprising a polynucleotide operably linked to at least one regulatory element, wherein said polynucleotide encodes a polypeptide having an amino acid sequence of at least 95% sequence identity when compared to SEQ ID NO: 36 or 38, wherein said dicot seed has an altered oxidoreductase motif (ORM) protein activity and altered oil, protein, starch and/or soluble carbohydrate content when compared to a seed from a control plant not comprising said polynucleotide.

16. The transformed seed of claim 15 , wherein said seed has an increased oil content of at least 2% when compared to the oil content of the control seed.

17. The seed of claim 15 , wherein the seed is soybean seed.

18. The transformed seed of claim 15 , wherein the transformed seed has increased oil content compared with the oil content of the control seed.

19. The transformed seed of claim 18 , wherein the transformed seed is a soybean seed.

20. A method for producing transgenic dicot seeds, the method comprising:
(a) transforming a plant cell with a recombinant DNA construct comprising (i) a polynucleotide operably linked to at least one regulatory sequence, wherein the polynucleotide encodes a polypeptide having an amino acid sequence of at least 95% sequence identity to SEQ ID NO: 36 or 38, or (ii) a full-length complement of the polynucleotide sufficient to reduce expression of endogenous oxidoreductase motif (ORM) protein in the plant cell;
(b) regenerating a transgenic plant from the transformed plant cell of (a); and
(c) selecting a transgenic dicot plant that produces a transgenic seed having an altered oil, protein, starch or soluble carbohydrate content as compared to a control seed obtained from a control plant not comprising said polynucleotide.

21. The method of any one of claim 1 , 6 , or 20 , wherein the seed is soybean seed.

22. The method of claim 20 , wherein the at least one regulatory element is a seed-specific or seed-preferred promoter.

23. Seed obtained by the method of claim 20 , wherein the seed is soybean seed, comprises the recombinant construct and exhibits an altered oil or protein content when compared to a seed from a control plant not comprising said polynucleotide.

24. The seed of claim 23 , wherein the seed exhibits an increase in oil content when compared to a seed from a control plant not comprising said polynucleotide.

Assignees

• Du Pont

Inventors

• Meyer Knut
• Stecca Kevin L

Classifications

• C12N15/8247

  involving modified lipid metabolism, e.g. seed oil composition · CPC title

• C12N9/0004 Primary

  Oxidoreductases (1.) · CPC title

• C12N15/8245

  involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis · CPC title

• C12N5/14

  Plant cells · CPC title

• C12N15/8251 Primary

  Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis · CPC title