Polypeptides having glucoamylase activity and polynucleotides encoding same

Patent metadata

Field	Value
Publication number	US-9617527-B2
Application number	US-201113637826-A
Country	US
Kind code	B2
Filing date	Apr 11, 2011
Priority date	Apr 14, 2010
Publication date	Apr 11, 2017
Grant date	Apr 11, 2017

Abstract

The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

First claim

What is claimed is:

1. A process of producing a saccharified material, comprising saccharifying a starch with a polypeptide having glucoamylase activity and at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2.

2. A process of producing a fermentation product, comprising
(a) liquefying a starch material to produce a liquefied starch material;
(b) saccharifying the liquefied starch material with a polypeptide having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2 to produce a sugar; and
(c) fermenting the sugar to produce the fermentation product.

3. The process of claim 2 , further comprising recovering the fermentation product.

4. The process of claim 3 , wherein the fermentation product is selected from the group consisting of alcohols, organic acids, ketones, amino acids; alkanes, cycloalkanes, alkenes, gases, antibiotics, enzymes, vitamins, and hormones.

5. A nucleic acid construct comprising a polynucleotide comprising a nucleic acid sequence that encodes a polypeptide having glucoamylase activity and at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2 operably linked to one or more heterologous control sequences that direct the production of the polypeptide in an expression host.

6. A recombinant expression vector comprising the nucleic acid construct of claim 5 .

7. A recombinant host cell comprising the nucleic acid construct of claim 5 .

8. A method of producing a polypeptide having glucoamylase activity and at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2, comprising:
(a) cultivating a host cell comprising a nucleic acid construct comprising a polynucleotide encoding the polypeptide under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.

9. A transgenic plant, plant part or plant cell transformed with a polynucleotide encoding a polypeptide having glucoamylase activity and at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2.

10. The process of claim 1 , wherein polypeptide has at least 96% sequence identity to the mature polypeptide of SEQ ID NO: 2.

11. The process of claim 1 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 2.

12. The process of claim 1 , wherein the polypeptide comprises the amino acid sequence of the mature polypeptide of SEQ ID NO: 2.

13. A process of producing a saccharified material comprising saccharifying a starch with a fragment of a polypeptide having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2, wherein the fragment has glucoamylase activity.

14. A composition comprising a polypeptide having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2, and an alpha-amylase, wherein the polypeptide has glucoamylase activity and the alpha-amylase has alpha-amylase activity.

15. A process for producing a liquefaction, saccharification and/or fermentation product from starch-containing material comprising treating starch-containing material with a polypeptide having glucoamylase activity and at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2.

16. The process of claim 15 , wherein an alpha-amylase is added.

17. The process of claim 15 , wherein the treating is carried out at a temperature between 40° C. and 100° C. and/or at a pH between 2.0 and 7.0.

Assignees

• Li Ming
• Duan Junxin
• Liu Zheng
• Fukuyama Shiro
• Ayabe Keiichi
• Coward-Kelly Guillermo
• Deinhammer Randall
• Novozymes As

Inventors

• Li Ming
• Duan Junxin
• Liu Zheng
• Fukuyama Shiro
• Ayabe Keiichi
• Coward-Kelly Guillermo
• Deinhammer Randall

Classifications

• Y02E50/17

  Cross-Sectional Technologies · mapped topic

• C12P2203/00

  Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source · CPC title

• C13K1/06

  obtained by saccharification of starch or raw materials containing starch · CPC title

• C12N9/2428 Primary

  Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase · CPC title

• C12P19/02

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title