Method for detecting mutation in target base sequence of nucleic acid, method for selectively inhibiting amplification of nucleic acid, and kits for performing same
US-2025043335-A1 · Feb 6, 2025 · US
US9617388B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9617388-B2 |
| Application number | US-201615282074-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 30, 2016 |
| Priority date | Dec 30, 2013 |
| Publication date | Apr 11, 2017 |
| Grant date | Apr 11, 2017 |
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A zwitterionic monomer and corresponding cyclopolymerized polyzwitterion (±) (PZ) (i.e. poly(Z-alt-SO 2 ). Phosophonate ester hydroloysis in PZ gave a pH-responsive polyzwitterionic acid (±) (PZA). The PZA under pH-induced transformation was converted into polyzwitterion/anion (±−) (PZAN) and polyzwitterion/dianion (±=) (PZDAN).
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The invention claimed is: 1. A process for making a polyzwitterion or polyzwitterionic acid having the following formula: where n is an integer of 10 or greater, the process comprising: copolymerizing a monomer of formula (I) with SO 2 in the presence of a polymerizing agent, wherein R is a C 1 to C 6 alkyl group or a C 6 -C 12 aryl group or an H group. 2. The process of claim 1 , wherein R is CH 2 CH 3 ; and the number of repeating units n is in the range of 20-1,500. 3. The process of claim 1 , wherein the polymerizing agent is tert-butyl hydroperoxide. 4. The process of claim 1 , further comprising: reacting the polyzwitterion with an acid solution to hydrolyze the P(O)(OR) 2 groups into groups of formula —P(O)(OH) 2 . 5. The process of claim 1 , wherein the polymerizing occurs in a solution of dimethylsulfoxide. 6. The process of claim 1 , wherein the polymerizing agent is azobisisobutyronitrile.
Polysulfones · CPC title
Use of other chemical agents · CPC title
Diallylamine · CPC title
Copolymers of sulfur dioxide with unsaturated organic compounds · CPC title
Copolymers of sulfur dioxide with unsaturated aliphatic compounds · CPC title
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