Polymicrobial formulations for enhancing plant productivity

Patent metadata

Field	Value
Publication number	US-9615584-B2
Application number	US-201414161424-A
Country	US
Kind code	B2
Filing date	Jan 22, 2014
Priority date	Jan 15, 2008
Publication date	Apr 11, 2017
Grant date	Apr 11, 2017

Abstract

Described herein are eco-friendly formulations and methods for providing such formulations to plants. The formulations include mixtures of microbial isolates. In particular, numerous bacterial and fungal strains were isolated from a variety of soil types, from rhizospheres and from root nodules of leguminous plants, in designed combinations, for providing plant growth and plant productivity enhancing formulations. These specifically designed polymicrobial formulations provide protection against plant pathogens, lower the need for nitrogen containing fertilizers, solubilize minerals, protect plants against pathogens, and make available to the plant valuable nutrients, such as phosphate, thus reducing and eliminating the need for using chemical fertilizers and chemical pesticides.

First claim

We claim:

1. A microbial formulation comprising a bacterial mixture of Ensifer meliloti FD, Rhizobium trifolii FD, Azorhizobium caulinodans KN, Rhizobium RLG1 , Azorhizobium sp. RLG2 , Azorhizobium sp. RLG3 , Rhizobium sp. RLG4 , Rhizobium sp. RLG5 , Rhizobium sp. RLG6 , Azorhizobium RLG7 , Rhizobium sp. RLG8 , Azorhizobium sp. RLG9 , Rhizobium sp. RLG10, and Rhizobium sp. RLG11 having N.R.R.L accession number B-50215 and a carrier.

2. The microbial formulation of claim 1 , wherein the carrier is a liquid carrier.

3. The microbial formulation of claim 2 , wherein said liquid carrier comprises water and humic acid.

4. The microbial formulation of claim 3 , wherein said humic acid is at a concentration of 12% volume of humic acid per volume of the formulation.

5. The microbial formulation of claim 2 , wherein said liquid carrier has a pH of 7.0.

6. The microbial formulation of claim 2 , wherein the bacterial mixture has a concentration in said liquid carrier that ranges from 10 10 -10 17 bacteria per milliliter of said liquid.

7. The microbial formulation of claim 1 , wherein the formulation is in a form selected from the group consisting of a liquid, a dried formulation, a wettable powder, and a freeze-dried liquid formulation.

8. The microbial formulation of claim 1 , further comprising an adjuvant selected from the group consisting of a wetting agent, spreading agent, dispersing agent, sticking agent, and adhesive.

9. The microbial formulation of claim 1 , wherein said formulation is a biocontrol formulation for a disease selected from the group consisting of leaf spot of tomato and powdery mildew.

10. The microbial formulation of claim 9 , wherein said leaf spot disease pathogen comprises a Curvularia species and an Alternaria species.

Assignees

• Univ Michigan State

Inventors

• Reddy C Adinarayana
• Janarthanam Lalithakumari

Classifications

• C12N1/14

  Fungi (culture of mushrooms A01G18/00; as new plants A01H15/00); Culture media therefor · CPC title

• C12N1/00

  Microorganisms; Compositions thereof (medicinal preparations containing material from protozoa, bacteria or viruses A61K35/66, from algae A61K36/02, from fungi A61K36/06; preparing medicinal bacterial antigen or antibody compositions, e.g. bacterial vaccines, A61K39/00); Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor · CPC title

• C05F11/08

  Organic fertilisers containing added bacterial cultures, mycelia or the like · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• Y02P60/218

  Cross-Sectional Technologies · mapped topic