Inoculants including Bacillus bacteria for inducing production of volatile organic compounds in plants

Patent metadata

Field	Value
Publication number	US-9615583-B2
Application number	US-201615014397-A
Country	US
Kind code	B2
Filing date	Feb 3, 2016
Priority date	Dec 10, 2010
Publication date	Apr 11, 2017
Grant date	Apr 11, 2017

Abstract

Disclosed are inoculants that include Bacillus bacteria and induce production of volatile organic compounds (VOCs) by a plant that has been treated with the inoculant.

First claim

We claim:

1. A composition for treating a plant or seeds of a plant, the composition formulated as a treatment composition in which are combined:
(a) an isolated Bacillus bacteria selected from the group consisting of:
(i) Bacillus amyloliquefaciens selected from the group consisting of Bacillus amyloliquefaciens strain AP-136 (NRRL B-50614), Bacillus amyloliquefaciens strain AP-188 (NRRL B-50615), Bacillus amyloliquefaciens strain AP-218 (NRRL B-50618), Bacillus amyloliquefaciens strain AP-219 (NRRL B-50619), and Bacillus amyloliquefaciens strain AP-295 (NRRL B-50620);
(ii) Bacillus mojavensis strain AP-209 (NRRL B-50616);
(iii) Bacillus solisalsi strain AP-217 (NRRL B-50617);
(iv) Bacillus simplex strain ABU 288 (NRRL B-50340); and
(v) mixtures thereof; and

(b) a suitable carrier;
the composition comprising 10 2 -10 12 cfu per ml carrier, and the composition inducing production of one or more volatile organic compounds (VOCs) by a plant that has been treated with the composition or that is grown from seed that has been treated with the composition.

2. The composition according to claim 1 , wherein the bacteria are selected from a group consisting of Bacillus amyloliquefaciens strain AP-136 (NRRL B-50614), Bacillus amyloliquefaciens strain AP-188 (NRRL B-50615), Bacillus amyloliquefaciens strain AP-218 (NRRL B-50618), Bacillus amyloliquefaciens strain AP-219 (NRRL B-50619), and Bacillus amyloliquefaciens strain AP-295 (NRRL B-50620).

3. The composition according to claim 1 , wherein the bacteria are Bacillus mojavensis strain AP-209 (NRRL B-50616).

4. The composition according to claim 1 , wherein the bacteria are Bacillus solisalsi strain AP-217 (NRRL B-50617).

5. The composition according to claim 1 , wherein the bacteria are Bacillus simplex strain ABU 288 (NRRL B-50340).

6. The composition according to claim 1 , wherein the carrier is selected from the group consisting of peat, wheat, bran, vermiculite, and pasteurized soil.

7. The composition according to claim 1 , wherein the one or more VOCs comprise one or more compounds selected from a group consisting of alpha-pinene, beta-pinene, beta-myrcene, cis-3-hexenyl acetate, limonene, beta-ocimene, linalool, (E)-4,8-dimethyl-1,3,7-nonatriene, methyl salicylate, decanal, cis-jasmone, caryophyllene, alpha-humulene, beta-farnesene, and mixtures thereof.

8. The composition according to claim 1 , wherein one or more VOCs reduce egg-laying of an insect on the plant.

9. The composition according to claim 1 , wherein one or more VOCs reduce feeding of an insect on the plant.

10. The composition according to claim 1 , wherein one or more VOCs attract a predator or a parasitoid to the plant.

11. A method of treating a plant, the method comprising administering the composition of claim 1 to the plant or to soil surrounding the plant.

12. The method according to claim 11 , wherein the plant is selected from the group consisting of alfalfa, rice, barley, rye, cotton, sunflower, peanut, corn, potato, sweet potato, bean, pea, lentil chicory, lettuce, endive, cabbage, brussel sprout, beet, parsnip, turnip, cauliflower, broccoli, turnip, radish, spinach, onion, garlic, eggplant pepper, celery, carrot, squash, pumpkin, zucchini, cucumber, apple, pear, melon, citrus, strawberry, grape, raspberry, pineapple, soybean, canola, oil seed rape, spring wheat, winter wheat, tobacco, tomato, sorghum, and sugarcane.

13. The method according to claim 11 , wherein the method reduces egg-laying of an insect on the plant.

14. The method according to claim 11 , wherein the method reduces feeding of an insect on the plant.

15. The method according to claim 11 , wherein the method attracts a predator insect or parasitoid to the plant.

16. A method of treating a plant seed, the method comprising treating the plant seed with the composition of claim 1 .

Assignees

• Univ Auburn

Inventors

• Kloepper Joseph W
• Fadamiro Henry Y
• Ngumbi Esther N
• Nangle Kate W

Classifications

• A01N63/00 Primary

  Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates (containing compounds of determined constitution A01N27/00 - A01N59/00; unicellular algae A01N65/03) · CPC title

• C12N1/20 Primary

  Bacteria; Culture media therefor · CPC title

• A01N2300/00

  Combinations or mixtures of active ingredients covered by classes A01N27/00 - A01N65/48 with other active or formulation relevant ingredients, e.g. specific carrier materials or surfactants, covered by classes A01N25/00 - A01N65/48 · CPC title

• C12R1/07

  Chemistry & Metallurgy · mapped topic

• A01N63/22 Primary

  Bacillus · CPC title