High-efficiency separation and manipulation of particles and cells in microfluidic device using surface acoustic waves at an oblique angle

Patent metadata

Field	Value
Publication number	US-9606086-B2
Application number	US-201313955709-A
Country	US
Kind code	B2
Filing date	Jul 31, 2013
Priority date	Aug 1, 2012
Publication date	Mar 28, 2017
Grant date	Mar 28, 2017

Abstract

An apparatus for manipulating particles within a fluid sample includes a substrate having a substrate surface. A surface acoustic wave (SAW) generator generates a SAW within a SAW region of the substrate surface. The SAW has an SAW direction aligned with a pressure node. A channel is configured to receive the fluid sample and the fluid sample has a flow direction which is at an oblique angle to the SAW direction.

First claim

The invention claimed is:

1. An apparatus for sorting particles within a fluid sample, the apparatus comprising:
a substrate, having a substrate surface;
a surface acoustic wave (SAW) generator configured to generate a SAW within a SAW region of the substrate surface, the SAW having a SAW direction aligned with a generally linear pressure node;
a channel, configured to receive the fluid sample, the fluid sample having a flow direction, the flow direction being at an oblique angle to the SAW direction; and
a plurality of output channels arranged and disposed to collect a plurality of output particle streams from the fluid sample in the channel.

2. The apparatus of claim 1 , wherein:
the SAW generator comprises a pair of spaced apart surface acoustic wave generators, the surface acoustic wave generators each being an interdigitated transducer including interdigitated electrodes supported by the substrate.

3. The apparatus of claim 1 , wherein:
the substrate is a piezoelectric substrate.

4. The apparatus of claim 1 , wherein:
the apparatus is a microfluidic device;
the channel being a microchannel having at least one cross-sectional dimension less than 1 mm; and
the particles being microparticles having a cross-sectional dimension less than 100 microns.

5. The apparatus of claim 1 , wherein:
the SAW generator is a standing surface acoustic wave (SSAW) generator configured to generate a SSAW within the SAW region.

6. An apparatus for sorting particles within a fluid sample, the apparatus comprising:
a substrate;
a first surface acoustic wave generator;
a second surface acoustic wave generator, the first and second surface acoustic wave generators being configured to generate a surface acoustic wave (SAW) within a SAW region of the substrate, the SAW having a SAW direction aligned with a generally linear pressure node of the SAW;
a channel configured to receive a fluid sample including particles, the channel having a sorting portion proximate the SAW region of the substrate, the channel having a channel direction; and
a plurality of output channels arranged and disposed to collect a plurality of output particle streams from the fluid sample in the channel,
wherein the SAW direction is disposed at an oblique angle to the channel direction, and
wherein by the apparatus is configured to sort particles within the fluid sample into the plurality of output particle streams when the fluid sample is introduced into the channel and the SAW is generated.

7. The apparatus of claim 6 , wherein:
the SAW generator is a standing surface acoustic wave (SSAW) generator configured to generate a SSAW within the SAW region.

8. The apparatus of claim 6 , wherein:
the substrate is a piezoelectric substrate; and
the first and second surface acoustic wave generators each comprise electrodes supported by the substrate.

9. The apparatus of claim 6 , wherein:
the substrate forms a wall of the channel.

10. The apparatus of claim 6 , wherein:
the apparatus is a microfluidic device;
the channel is a microchannel; and
the microchannel has at least one cross-sectional dimension less than 1 mm.

11. The apparatus of claim 6 , wherein:
the channel is a flow channel configured to receive a sample fluid flow.

12. The apparatus of claim 6 , wherein:
the particles are microparticles having a diameter of less than 100 microns.

13. The apparatus of claim 12 , wherein:
the microparticles include biomolecules or cells.

14. A method of sorting a plurality of different types of particles within a fluid sample including the plurality of different types of particles, the method comprising:
providing an apparatus for sorting particles within a fluid sample, the apparatus comprising:
a substrate, having a substrate surface;
a surface acoustic wave (SAW) generator;
a channel, configured to receive the fluid sample; and
a plurality of output channels;

introducing the fluid sample to the flow channel, the fluid sample including the plurality of different types of particles and having a flow direction;
generating a SAW within a SAW region of the substrate surface, the SAW having a SAW direction aligned with a generally linear pressure node, the flow direction being at an oblique angle to the SAW direction;
sorting the plurality of different types of particles into a plurality of output particle streams by type; and
sorting the plurality of output particle streams into the plurality of output channels by type.

15. The method of claim 14 , wherein:
the generating a SAW step comprises generating a standing surface acoustic wave (SSAW).

16. The method of claim 14 , wherein:
the fluid sample is a sample flow directed along the flow channel;
the flow channel is supported by the substrate;
the flow channel is a microchannel within a microfluidic device; and
the method further includes particle characterization, particle focusing, particle separation, particle fractionation, or particle selection.

17. The method of claim 14 , wherein:
the fluid sample includes a first particle type being directed by pressure forces induced by the SAW and a second particle type not being directed by the pressure forces, so as to produce a first stream of the first particle type and a second stream of the second particle type.

Assignees

• Penn State Res Found

Inventors

• Ding Xiaoyun
• Huang Tony Jun

Classifications

• B01L3/502761 Primary

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title

• B01L2400/0436

  acoustic forces, e.g. surface acoustic waves [SAW] · CPC title

• G01N29/02 Primary

  Analysing fluids (using acoustic emission techniques G01N29/14 {; constructional or flow details for analysing fluids G01N29/222; optoacoustic fluid cells G01N29/2425}) · CPC title

• G01N15/1459

  the analysis being performed on a sample stream · CPC title

• G01N2015/0019

  Means for transferring or separating particles prior to analysis, e.g. hoppers or particle conveyors · CPC title