Methods and compositions to enhance plant breeding

Patent metadata

Field	Value
Publication number	US-9605272-B2
Application number	US-201414283630-A
Country	US
Kind code	B2
Filing date	May 21, 2014
Priority date	May 27, 2005
Publication date	Mar 28, 2017
Grant date	Mar 28, 2017

Abstract

The present invention provides breeding methods and compositions to enhance the germplasm of a plant. The methods describe the identification and accumulation of transgenes and favorable haplotype genomic regions in the germplasm of a breeding population of crop plants.

First claim

What is claimed is:

1. A method of breeding a transgenic corn plant comprising the steps of:
providing at least two transgenic corn plants, each corn plant having at least one transgene inserted into its genome;
determining a map location of the at least one transgene in the genome of the at least two transgenic corn plants using at least one DNA marker in the genomic region flanking the transgene insert; and
selecting at least one of the transgenic corn plants for breeding, wherein the at least one transgenic corn plant that is selected has in its genome the at least one transgene that is genetically linked to haplotype C8W4H5; and
crossing the selected at least one transgenic corn plants with a second corn plant to produce one or more progeny plants comprising the at least one transgene linked to haplotype C8W4H5; and
wherein the at least one transgene and the haplotype are linked at a genetic distance of 0 to within about 5 cM.

2. The method of claim 1 , wherein the method further comprises selecting a progeny plant of the at least one transgenic corn plant selected for breeding by marker-assisted selection.

3. The method of claim 1 , wherein the method further comprises selecting a progeny plant of the at least one transgenic corn plant selected for breeding by detection of expression of the at least one transgene or expression of a transgenic agronomic trait.

4. The method of claim 1 , wherein the at least one DNA marker selected from the group consisting of SEQ ID NO: 51-54.

5. The method of claim 3 , further comprising the step of crossing the progeny plant with another corn plant to produce additional progeny plants.

6. The method of claim 1 , wherein the at least one transgene encodes a protein providing an agronomic enhancement selected from the group consisting of herbicide tolerance, disease resistance, insect or pest resistance, altered fatty acid, protein or carbohydrate metabolism, increased grain yield, increased oil, altered plant maturity, enhanced stress tolerance, and altered morphological characteristics.

7. The method of claim 6 , wherein the herbicide tolerance is selected from the group consisting of glyphosate, glufosinate, sulfonylureas, imidazolinones, bromoxynil, dalapon, dicamba, 2,4-D, cyclohezanedione, protoporphyrinogen oxidase inhibitors, and isoxaflutole tolerance.

Assignees

• Monsanto Technology Llc

Inventors

• Bull Jason
• Butruille David
• Eathington Sam
• Edwards Marlin
• Gupta Anju
• Johnson Richard
• Kennard Wayne
• Rinehart Jennifer
• Wu Kunsheng

Classifications

• C12N15/8275 Primary

  Glyphosate · CPC title

• A01N57/20

  containing acyclic or cycloaliphatic radicals · CPC title

• C12N15/821

  Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers · CPC title

• C12N15/8286

  for insect resistance · CPC title

• A01H1/02

  Methods or apparatus for hybridisation; Artificial pollination {; Fertility} · CPC title