Electrokinetic confinement of neurite growth for dynamically configurable neural networks

Patent metadata

Field	Value
Publication number	US-9605253-B2
Application number	US-201414154797-A
Country	US
Kind code	B2
Filing date	Jan 14, 2014
Priority date	Jan 14, 2013
Publication date	Mar 28, 2017
Grant date	Mar 28, 2017

Abstract

Systems and methods for altering neurite growth are generally described. In some embodiments, a system may include a neuron comprising a neurite and electrodes able to generate a physical guidance cue. The physical guidance cue may be used to alter the growth of the neurite and may be temporally and spatially dynamic, such that neurite growth may be altered in a spatial and/or temporal manner. Dynamic control of neurite growth may be used to form directional neural connections, intersections, and/or overlaps.

First claim

What is claimed is:

1. A method, comprising:
providing a neuron comprising one or more neurites;
providing an alternating current electric field; and
positioning the alternating current electric field on an elongation field of the neurites to directionally guide elongation by repelling neurite growth in a region of the alternating current electric field.

2. A method according to claim 1 , comprising:
providing a neuron comprising a neurite;
providing an alternating current electric field, wherein the alternating current electric field can reversibly arrest growth of the neurite; and
controlling growth of the neurite using the alternating current electric field.

3. A method according to claim 2 , comprising:
providing more than one neuron, wherein each neuron comprises one or more neurites;
controlling a neurite independently of another neurite; and
forming a neural network from the more than one neuron.

4. A method as in claim 3 , wherein the electric field is non-uniform.

5. A method according to claim 1 , comprising:
providing more than one neuron, wherein each neuron comprises one or more neurites;
controlling a neurite independently of another neurite; and
forming a neural network from the more than one neuron.

6. A method as in claim 5 , wherein the electric field is non-uniform.

7. A method as in claim 1 , comprising providing the alternating electric field with a magnitude and a frequency to form a unidirectional neuronal connection.

8. A method as in claim 1 , comprising providing the alternating electric field with a magnitude and a frequency to form an axon diode.

9. A method as in claim 1 , wherein the electric field can be controlled to turn on or off.

10. A method as in claim 1 , comprising guiding neurite elongation in two dimensions.

11. A method as in claim 1 , comprising guiding neurite elongation in three dimensions.

12. A method as in claim 1 , comprising providing the neuron in a channel and inhibiting elongation to the channel, where the magnitude of the electric field in the channel is less than 100 V/m.

13. A method as in claim 1 , comprising guiding elongation of the neurite by changing the electric field.

14. A method as in claim 1 , wherein the magnitude of the electric field is greater than or equal to about 100 V/m.

15. A method as in claim 1 , wherein the frequency of the electric field is greater than or equal to about 100 Hz.

16. A method as in claim 1 , wherein the field is produced by two or more electrodes having a center to center spacing between the electrodes of less than or equal to about 200 microns.

17. A method as in claim 1 , wherein the neuron is a hippocampus neuron.

18. A method as in claim 1 , wherein the neurite is an axon.

Assignees

• Centre Nat Rech Scient
• Massachusetts Inst Technology

Inventors

• Voldman Joel
• Honegger Thibault
• Peyrade David

Classifications

• C12M21/08

  for producing artificial tissue or for ex-vivo cultivation of tissue (prostheses A61F2/00, grafts A61L27/00) · CPC title

• B03C5/005

  Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength · CPC title

• C12N2529/00

  Culture process characterised by the use of electromagnetic stimulation · CPC title

• A61N1/326

  for promoting growth of cells, e.g. bone cells · CPC title

• B03C2201/26

  for use in medical or biological applications · CPC title