Scaffolds For Cell Transplantation
US-2015024026-A1 · Jan 22, 2015 · US
Materials presenting notch signaling molecules to control cell behavior
US9603894B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9603894-B2 |
| Application number | US-201113883005-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 8, 2011 |
| Priority date | Nov 8, 2010 |
| Publication date | Mar 28, 2017 |
| Grant date | Mar 28, 2017 |
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- Title
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Abstract
Official abstract text for this publication.
The invention provides a solution to the problem of delivering molecules in a physiologically relevant manner to direct cell fate. For example, a Notch ligand molecule is presented in a microenvironment that mimics the environment encountered in vivo. Accordingly, the invention features a cell delivery vehicle comprising a biocompatible hydrogel or polymer and a composition that binds to a Notch receptor and methods of directing cell fate, e.g., stem cell differentiation, using such compositions.
First claim
Opening claim text (preview).
What is claimed is: 1. A method of inducing differentiation of a population of mesenchymal stem cells comprising transiently contacting the population of mesenchymal stem cells with: a) a biocompatible alginate hydrogel covalently linked to human Jagged-1, said Jagged-1 comprising the amino acid sequence of SEQ ID NO:1, or a fragment thereof, said fragment comprising the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:3, and b) a bone morphogenetic protein, thereby inducing differentiation of the population of mesenchymal stem cells into a population of cells of osteogenic lineage, wherein the level of alkaline phosphatase activity of the population of cells after said contacting is increased by at least 4-fold as compared to the level of alkaline phosphatase activity of a population of mesenchymal stem cells that has been contacted with the bone morphogenetic protein but not the alginate hydrogel. 2. The method of claim 1 , wherein said bone morphogenetic protein is bone morphogenetic protein-4. 3. The method of claim 1 , wherein said population of cells of osteogenic lineage comprises bone cells or cartilage cells. 4. The method of claim 2 , wherein said bone morphogenetic protein-4 comprises amino acids 36-275 or 308-408 of SEQ ID NO:4. 5. The method of claim 1 , wherein said alginate hydrogel comprises alginate covalently linked to a peptide molecule comprising an arginine-glycine-aspartic acid (RGD) sequence.
Assignees
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Classifications
from mammals · CPC title
Human Necessities · mapped topic
- A61K38/10Primary
Peptides having 12 to 20 amino acids {(A61K38/043 - A61K38/046 take precedence)} · CPC title
Human Necessities · mapped topic
the organic macromolecular compound being a polysaccharide or a derivative thereof · CPC title
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- What does patent US9603894B2 cover?
- The invention provides a solution to the problem of delivering molecules in a physiologically relevant manner to direct cell fate. For example, a Notch ligand molecule is presented in a microenvironment that mimics the environment encountered in vivo. Accordingly, the invention features a cell delivery vehicle comprising a biocompatible hydrogel or polymer and a composition that binds to a Notc…
- Who is the assignee on this patent?
- Cao Lan, Bouhadir Kamal H, Mooney David J, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification A61K38/10. Mapped technology areas include Human Necessities.
- When was this patent published?
- Publication date Tue Mar 28 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 2 related publications on this page (citations in our corpus or others sharing the same primary CPC).