Polypeptides having α-xylosidase activity and polynucleotides encoding same

Patent metadata

Field	Value
Publication number	US-9603378-B2
Application number	US-201414896871-A
Country	US
Kind code	B2
Filing date	Jul 25, 2014
Priority date	Jul 26, 2013
Publication date	Mar 28, 2017
Grant date	Mar 28, 2017

Abstract

The present invention relates to isolated polypeptides having α-xylosidase activity, catalytic domains and polynucleotides encoding the polypeptides, catalytic domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides and catalytic domains.

First claim

The invention claimed is:

1. An animal feed additive comprising:
(a) at least one polypeptide having alpha-xylosidase activity, wherein the polypeptide has at least 90% sequence identity to the sequence of amino acids 1-793 of SEQ ID NO: 2; and
(b) at least one fat-soluble vitamin, at least one water-soluble vitamin, or at least one trace mineral.

2. The animal feed additive of claim 1 , which comprises at least one fat-soluble vitamin, at least one water-soluble vitamin, and at least one trace mineral.

3. The animal feed additive of claim 1 , wherein the polypeptide has at least 95% sequence identity to the sequence of amino acids 1-793 of SEQ ID NO: 2.

4. The animal feed additive of claim 1 , wherein the polypeptide has at least 97% sequence identity to the sequence of amino acids 1-793 of SEQ ID NO: 2.

5. The animal feed additive of claim 1 , wherein the polypeptide is a fragment of the sequence of amino acids 1-793 of SEQ ID NO: 2, and has α-xylosidase activity.

6. The animal feed additive of claim 1 , wherein the polypeptide comprises the sequence of amino acids 1-793 of SEQ ID NO: 2.

7. The animal feed additive of claim 1 , wherein the polypeptide is in the form of a granulate or microgranulate.

8. An animal feed comprising an animal or vegetable protein and the animal feed additive of claim 1 .

9. The animal feed of claim 8 , wherein the animal protein is selected from the group consisting of meat meal, bone meal, feather meal, fish meal, and mixtures thereof.

10. The animal feed of claim 8 , wherein the vegetable protein is a legume or a cereal.

11. The animal feed of claim 8 , wherein the vegetable protein is soy bean meal, lupin meal, rapeseed meal, soybean, lupine, pea, bean, beet, sugar beet, spinach, quinoa, rapeseed, sunflower seed, cotton seed, or cabbage.

12. The animal feed of claim 8 , further comprising dried distillers grains with solubles.

13. The animal feed of claim 8 , further comprising maize; sorghum; wheat; barley; oats; soybean meal; fish meal; meat and bone meal; and/or whey.

14. The animal feed of claim 8 , which is in the form of mash feed or pelleted feed.

15. The animal feed of claim 8 , further comprising a GH95 enzyme.

16. The animal feed of claim 8 , further comprising GH10 and GH11 enzymes.

17. The animal feed of claim 8 , further comprising GH43 and GH51 enzymes.

18. A method for feeding an animal, comprising feeding the animal the animal feed of claim 9 .

19. The method of claim 18 , wherein the animal is selected from the group consisting of pigs or swine; poultry; horses, fish; crustaceans; sheep; goats; and cattle.

Assignees

• Novozymes As

Inventors

• Krogh Kristian Bertel Romer M
• Eklof Jens Magnus
• Johansen Katja Salomon
• Rogowski Arthur
• Bolam David N
• Gilbert Harry J

Classifications

• A23K20/20

  Inorganic substances, e.g. oligoelements · CPC title

• A23K20/189 Primary

  Enzymes · CPC title

• A23K20/142

  Amino acids; Derivatives thereof · CPC title

• A23K20/147

  Polymeric derivatives, e.g. peptides or proteins · CPC title

• A23K10/14

  Pretreatment of feeding-stuffs with enzymes · CPC title