Flow chambers, methods of using the flow chamber, and methods of making the flow chamber

Patent metadata

Field	Value
Publication number	US-9599604-B2
Application number	US-201214281186-A
Country	US
Kind code	B2
Filing date	Dec 7, 2012
Priority date	Dec 9, 2011
Publication date	Mar 21, 2017
Grant date	Mar 21, 2017

Abstract

In accordance with the purpose(s) of the present disclosure, as embodied and broadly described herein, embodiments of the present disclosure, in one aspect, relate to a device (or “flow chamber”), methods of making a device, methods of using a device, and the like.

First claim

I claim:

1. A device comprising:
a first plate and a second plate defining a flow chamber between the plates, the first plate configured to hold an independent substrate comprising a sample and comprising a securing structure for securing the substrate within the flow chamber, and the second plate comprising an integrated, recessed, optically transparent viewing window, a top surface of the viewing window being recessed below a top surface of the second plate and defining a recessed area in the second plate, and a bottom surface of the viewing window forming a portion of the flow chamber adjacent the sample to allow viewing of the sample through the viewing window;
a first inlet port at a first end of the device that is configured to flow a first fluid;
a flow channel within the flow chamber defined in part by the second plate and in part by the first plate, the substrate, or both, wherein the flow channel is in fluidic communication with the first inlet port and is configured so the first fluid contacts a side of the sample adjacent the viewing window and the sample is viewed through the optically transparent viewing window in real time as the sample is exposed to the first fluid;
and an exit port that is in fluidic communication with the flow channel, wherein the flow channel is configured so that the first fluid flows from the first inlet port side to the exit port side of the flow channel.

2. The device of claim 1 , wherein the flow channel has a length of about 5 mm to 250 mm, a width of about 1 mm to 50 mm, and a height of about 25 μm to 10 mm.

3. The device of claim 1 , wherein the flow channel is configured so that the first fluid contacts both sides of the substrate.

4. The device of claim 1 , wherein the flow channel is configured so that the substrate defines one surface of the flow channel and the second plate defines a second surface of the flow channel and the first fluid contacts the side of the substrate adjacent the viewing window.

5. The device of claim 1 , wherein the securing structure includes a groove in the first plate that secures the substrate within the flow chamber.

6. The device of claim 5 , further comprising a second securing structure to secure the substrate to the groove, wherein the second securing structure is positioned on the side of the substrate opposite of the groove.

7. The device of claim 6 , wherein the second securing structure is a gasket configured to fit within the groove to secure a portion of the substrate within the groove.

8. The device of claim 1 , wherein the substrate comprises a flexible scaffold material including a plurality of cells.

9. The device of claim 1 , further comprising: a second inlet port at the first end of the device that is configured to flow a second fluid; wherein the flow channel is in fluidic communication with the second inlet port, wherein the flow channel is configured so the second fluid contacts a side of the sample.

10. The device of claim 9 , wherein the flow channel is configured so that the second fluid contacts both sides of the sample.

11. The device of claim 9 , wherein the flow channel is configured so that the second fluid contacts one side of the sample opposite the side of the sample contacted by the first fluid.

12. The device of claim 9 , further comprising an optically transparent second viewing window in the first plate disposed adjacent the flow channel on the side opposite the viewing window to view the sample in real time as the sample is exposed to the second fluid.

13. The, device of claim 1 , wherein the recessed, optically transparent viewing window is configured to allow positioning an objective of a microscope within the recessed area adjacent the top surface of the optically transparent viewing window to view the sample under magnification.

14. The device of claim 13 , wherein the microscope objective is positioned about 25 μm to 10 mm from the sample.

15. The device of claim 13 , wherein the optically transparent viewing window has a thickness adapted to allow imaging of the sample with a microscope under magnification of about 40× or greater.

16. The device of claim 1 , wherein the substrate is opaque.

17. The device of claim 1 , wherein the substrate has a thickness between 0.5 and 1.5 mm.

18. A flow chamber assembly comprising:
a base plate configured to hold a substrate comprising a sample, the base plate comprising a securing structure for securing the substrate within the flow chamber; and
a flow chamber plate configured to fit over the base plate, such that a first flow channel is defined by the base plate, substrate, and flow chamber plate, the flow chamber plate comprising:
a top surface;
a bottom surface configured to secure to the base plate;
a first inlet port at a first end of the flow chamber plate that is in fluidic communication with the flow channel and is configured to flow a first fluid such that the first fluid contacts a first surface of the substrate facing the flow chamber plate;
an exit port at a second end of the device that is in fluidic communication with the flow channel and is also configured to flow a first fluid; and
an optically transparent viewing window comprising a transparent material having a lower surface defining a portion of the flow channel above the substrate and having an upper surface recessed below the top surface of the flow chamber plate, wherein the optically transparent viewing window is configured to allow viewing of the substrate through the transparent material,

wherein the flow chamber plate is configured so that the first fluid flows from the first inlet port side, through the flow channel where it contacts the first surface of the substrate, to the exit port side of the flow channel, such that the substrate is viewed in real time as the substrate is exposed to the first fluid.

19. The device of claim 18 , wherein the securing structure on the base plate comprises a groove in the base plate for securing the substrate within the flow chamber, and wherein the device further comprises a gasket positioned on a side of the substrate opposite of the groove and configured to fit within the groove, wherein the gasket secures a portion of the substrate within the groove.

20. The device of claim 18 , wherein the substrate comprises a compliant vascular biomaterial.

Assignees

• Univ Florida

Inventors

• Mcfetridge Peter S

Classifications

• C12M29/10

  Perfusion · CPC title

• C12M23/22

  Transparent or translucent parts (glassware for laboratory use B01L3/00) · CPC title

• C12M25/04

  in combination with well or multiwell plates, i.e. culture inserts · CPC title

• C12M1/18

  Multiple fields or compartments · CPC title

• C12M23/46

  Means for fastening · CPC title