Mutant RB69 DNA polymerase

Patent metadata

Field	Value
Publication number	US-9593315-B2
Application number	US-201615200670-A
Country	US
Kind code	B2
Filing date	Jul 1, 2016
Priority date	Jun 5, 2009
Publication date	Mar 14, 2017
Grant date	Mar 14, 2017

Abstract

Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.

First claim

What is claimed:

1. An isolated nucleic acid comprising a nucleotide sequence encoding a mutant DNA polymerase having the amino acid sequence of SEQ ID NO:2.

2. A vector comprising the nucleic acid sequence of claim 1 .

3. The vector of claim 2 , further comprises a promoter sequence which is operably joined to the nucleic acid sequence encoding the mutant DNA polymerase.

4. The vector of claim 3 , wherein the promoter is constitutive or inducible.

5. A host cell carrying the vector of claim 2 .

6. A host cell carrying the vector of claim 3 .

7. A method for producing a mutant DNA polymerase polypeptide, comprising culturing the host cell of claim 6 under conditions suitable for the host cell to produce the mutant DNA polymerase polypeptide.

8. The mutant DNA polymerase produced by the method of claim 7 .

Assignees

• Life Technologies Corp

Inventors

• Peris Marian
• Phelan Michael
• Rosenblum Barnett
• Hendricks Stephen

Classifications

• C12Q1/6827

  for detection of mutation or polymorphism · CPC title

• C12N9/1252 Primary

  DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase · CPC title

• C12Q1/68

  involving nucleic acids · CPC title

• C12Q1/6869

  Methods for sequencing · CPC title

• C12Y207/07007

  DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase · CPC title