Bacterial colicin-immunity protein protein purification system
US-2024417426-A1 · Dec 19, 2024 · US
US9593158B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9593158-B2 |
| Application number | US-201314427659-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 16, 2013 |
| Priority date | Sep 18, 2012 |
| Publication date | Mar 14, 2017 |
| Grant date | Mar 14, 2017 |
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The present invention relates to a process for preparing gelatin comprising a step of incubating a material comprising collagen with an enzyme composition comprising an acid protease which has at least 70% identity to the amino acid sequence of SEQ ID NO:1, and preparing the gelatin. The invention also relates to gelatin obtained by a process as disclosed herein.
Opening claim text (preview).
The invention claimed is: 1. A process for preparing gelatin comprising: incubating a material comprising collagen with an enzyme composition comprising an acid protease to solubilize the collagen, wherein the acid protease has aspergillopepsin II protease activity and comprises an amino acid sequence which has at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 1, and preparing gelatin from the solubilized collagen, wherein the acid protease comprises at least 99% by weight of proteins in the enzyme composition, and wherein the yield of gelatin is at least 60% by weight of the material comprising collagen and the gelatin has a Bloom value of at least 200. 2. The process according to claim 1 , wherein the incubating is performed at a temperature of between 40 and 65 degrees Celsius. 3. The process according to claim 1 , wherein the incubating is performed at a pH of between 1.5 and 4.5. 4. The process according to claim 1 wherein the incubating is performed during a period of 1 to 10 hrs. 5. The process according to claim 1 wherein the acid protease is derived from an Aspergillus niger species. 6. The process according to claim 1 , further comprising separating solubilized collagen from insoluble collagen. 7. The process according to claim 1 further comprising drying the gelatin. 8. The process according to claim 1 , wherein the incubating is performed at a temperature of between 40 and 65 degrees Celsius, at a pH of between 1.5 and 4.5, and for a period of 1 to 10 hrs.
produced by the hydrolysis of a peptide bond, e.g. hydrolysate products (preparing foodstuffs by protein hydrolysis A23J3/00) · CPC title
using catalysts, e.g. selective catalysts · CPC title
from Aspergillus · CPC title
Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG] · CPC title
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