Substrates, systems and methods for analyzing materials

Patent metadata

Field	Value
Publication number	US-9587276-B2
Application number	US-201514945256-A
Country	US
Kind code	B2
Filing date	Nov 18, 2015
Priority date	Sep 1, 2006
Publication date	Mar 7, 2017
Grant date	Mar 7, 2017

Abstract

Substrates, systems and methods for analyzing materials that include waveguide arrays disposed upon or within the substrate such that evanescent fields emanating from the waveguides illuminate materials disposed upon or proximal to the surface of the substrate, permitting analysis of such materials. The substrates, systems and methods are used in a variety of analytical operations, including, inter alia, nucleic acid analysis, including hybridization and sequencing analyses, cellular analyses and other molecular analyses.

First claim

What is claimed is:

1. A method of performing a sequencing by synthesis reaction, the method comprising:
a) providing a substrate comprising:
i) a first surface and a mask layer disposed over the first surface of the substrate, the mask layer comprising a plurality of apertures that pass through the mask layer, wherein the apertures are configured to spatially confine an individual, optically resolvable complex;
ii) at least a first optical waveguide disposed beneath but sufficiently proximal to the plurality of apertures that when excitation radiation is passed through the first optical waveguide, an evanescent field emanating from the first optical waveguide reaches into the apertures;

b) immobilizing an individual polymerase/template/primer complex within one of the apertures sufficiently proximal to the first optical waveguide to be illuminated by the evanescent field;
c) passing excitation light through the first optical waveguide, thereby generating the evanescent field;
d) exposing the polymerase/template/primer complex to a set of labeled nucleotides or nucleotide analogs under conditions that promote nascent strand synthesis; and
e) detecting emissions from said one of the apertures, wherein said emissions are emitted from the labeled nucleotides or nucleotide analogs during said nascent strand synthesis, and are indicative of a sequence being synthesized, thereby performing a sequencing by synthesis reaction.

2. The method of claim 1 , wherein the labeled nucleotides or nucleotide analogs comprise fluorescent labels.

3. The method of claim 2 , wherein the fluorescent labels on the labeled nucleotides or nucleotide analogs are removed during said nascent strand synthesis.

4. The method of claim 1 , wherein the substrate further comprises at least one integrated optical functionality selected from the group consisting of mask layers, lenses, filters, gratings, antireflective coatings, optical switching components, or attenuation components.

5. The method of claim 1 , wherein said passing excitation light through the first optical waveguide comprises optically coupling at least a first excitation radiation source to the first optical waveguide.

6. The method of claim 1 , wherein said passing excitation light through the first optical waveguide comprises optically coupling at least two excitation radiation sources to the first optical waveguide.

7. The method of claim 6 , wherein the at least two excitation radiation sources provide additional intensity to the excitation light.

8. The method of claim 1 , wherein said mask layer comprises at least one material selected from the group consisting of a metal, a polymeric material, and a silica-based material.

9. The method of claim 1 , wherein said mask layer is a transparent mask layer.

10. The method of claim 1 , wherein the polymerase/template/primer complex comprises a tag sequence ligated to a template sequence and a primer annealed to the tag sequence.

11. A method for analyzing an analyte, comprising:
a) providing a substrate comprising:
i) a first surface;
ii) a mask layer disposed over the first surface, the mask layer comprising a plurality of apertures that pass through the mask layer; and
iii) at least a first optical waveguide disposed beneath but sufficiently proximal to at least a first aperture in the plurality of apertures that when excitation radiation is passed through the first optical waveguide, an evanescent field emanating from the first optical waveguide reaches into at least the first aperture;

b) disposing an optically resolvable individual analyte within said first aperture, wherein the analyte is disposed sufficiently proximal to the first optical waveguide to be illuminated by the evanescent field;
c) directing light into the first optical waveguide, thereby generating the evanescent field and, in doing so, illuminating the analyte; and
d) detecting an optical signal from the analyte disposed within said first aperture, thereby analyzing the analyte.

12. The method of claim 11 , wherein said directing light into the first optical waveguide excites a fluorescent moiety linked to the optically resolvable individual analyte, and further wherein said detecting an optical signal from the analyte comprises detecting a fluorescent signal from the fluorescent moiety.

13. The method of claim 11 , wherein the substrate comprises a plurality of optical waveguides disposed beneath but sufficiently proximal to the plurality of apertures such that when excitation radiation is passed through the plurality of optical waveguides, a plurality of evanescent fields emanating from the plurality of optical waveguide reaches into the plurality of apertures.

14. The method of claim 11 , wherein said directing light into the first optical waveguide comprises directing light from multiple light sources into the first optical waveguide.

15. The method of claim 11 , wherein the first optical waveguide is optically coupled to two or more originating waveguides disposed beneath the first surface.

16. The method of claim 11 , wherein the substrate further comprises at least one integrated optical functionality selected from the group consisting of mask layers, lenses, filters, gratings, antireflective coatings, optical switching components, or attenuation components.

17. The method of claim 11 , wherein the optically resolvable individual analyte is a binding partner for an immobilized binding agent in said one of the apertures.

18. The method of claim 11 , wherein said disposing the optically resolvable individual analyte comprises interaction of the optically resolvable individual analyte with an immobilized enzyme complex.

19. The method of claim 18 , wherein the optically resolvable individual analyte is a fluorescently labeled nucleotide and the immobilized enzyme complex is a polymerase/template/primer complex.

20. The method of claim 19 , wherein said first aperture contains only one polymerase/template/primer complex.

Assignees

• Pacific Biosciences California Inc

Inventors

• Lundquist Paul
• Turner Stephen

Classifications

• G01N2021/7786

  Fluorescence · CPC title

• B01L2300/0636

  Integrated biosensor, microarrays · CPC title

• G02B6/43

  Arrangements comprising a plurality of opto-electronic elements and associated optical interconnections · CPC title

• G01N21/645

  Specially adapted constructive features of fluorimeters · CPC title

• C12Q1/6869

  Methods for sequencing · CPC title