Non-sialylated anti-inflammatory polypeptides

Patent metadata

Field	Value
Publication number	US-9587025-B2
Application number	US-201214368701-A
Country	US
Kind code	B2
Filing date	Dec 10, 2012
Priority date	Dec 19, 2011
Publication date	Mar 7, 2017
Grant date	Mar 7, 2017

Abstract

This invention concerns anti-inflammatory agents, compositions, and methods for treating inflammatory disorders.

First claim

What is claimed:

1. An isolated polypeptide comprising a modified sequence that comprises SEQ ID NO: 2 and is at least 75% identical to the Fc region of an IgG (IgG Fc region), wherein the modified sequence is free of sialylation, and the polypeptide has an ability to bind to a Fc receptor and an anti-inflammatory activity that is higher than that of a parent polypeptide, said parent polypeptide comprising the IgG Fc region.

2. The isolated polypeptide of claim 1 , wherein the isolated polypeptide has an ability to bind to Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin (DC-SIGN).

3. The isolated polypeptide of claim 1 , wherein the isolated polypeptide has an ability to bind to hFcγRIIA or hFcγRIIB.

4. The isolated polypeptide of claim 3 , wherein the isolated polypeptide has an ability to bind to hFcγRIIA or hFcγRIIB at a K D of 2×10 −5 M or lower.

5. The isolated polypeptide of claim 1 , wherein the modified sequence consists essentially of SEQ ID NO: 2.

6. A pharmaceutical formulation comprising (i) the polypeptide of any of claims 1 , 2 - 4 , and 5 , and (ii) a pharmaceutically acceptable carrier.

7. A method of treating an inflammatory disease, comprising administering to a subject in need thereof a therapeutically effective amount of the polypeptide of any of claims 1 , 2 - 4 , and 5 .

Assignees

• Univ Rockefeller

Inventors

• Ravetch Jeffrey V
• Pincetic Andrew

Classifications

• A61P5/14

  of the thyroid hormones, e.g. T3, T4 · CPC title

• A61P37/06

  Immunosuppressants, e.g. drugs for graft rejection · CPC title

• A61P37/08

  Antiallergic agents (antiasthmatic agents A61P11/06; ophthalmic antiallergics A61P27/14) · CPC title

• A61P9/00

  Drugs for disorders of the cardiovascular system · CPC title

• A61P9/10

  for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis · CPC title