Media for affinity chromatography

Patent metadata

Field	Value
Publication number	US-9587009-B2
Application number	US-201414325014-A
Country	US
Kind code	B2
Filing date	Jul 7, 2014
Priority date	Jul 10, 2007
Publication date	Mar 7, 2017
Grant date	Mar 7, 2017

Abstract

The invention relates generally to solid supports for chromatography. In specific embodiments the invention provides for solid supports suitable for affinity chromatography along with methods, systems and kits which use the same.

First claim

What is claimed is:

1. A controlled porous glass bead comprising a pore size of about 839 Å; a mean particle size of 63 μm and an affinity ligand comprising Protein A linked to the bead, the affinity ligand having an affinity ligand density from 12 g/L to 14 g/L.

2. A controlled porous glass bead comprising a pore size of about 700 Å; a mean particle size of 55 μm±13 μm and an affinity ligand comprising Protein A linked to the bead, the affinity ligand having an affinity ligand density from 12 g/L to 14 g/L.

3. A method of isolating a target molecule comprising: a) contacting a mixture containing the target molecule with an affinity chromatography matrix where the matrix comprises 1) a solid support comprising a silica particle having a pore size greater than 630 Å and less than 1000 Å and a mean particle size greater than 55 μm and less than 70 μm and 2) an affinity ligand having specificity for the target molecule linked to the solid support, the affinity ligand having an affinity ligand density from 12 g/L to 14 g/L; and optionally b) eluting the target molecule from the affinity chromatography matrix.

4. The method of claim 3 , wherein the affinity chromatography matrix is contained in a housing and the contacting step comprises running the mixture containing the target molecule over the affinity chromatography matrix at a maximum flow rate ranging from 600 cm/hour to 900 cm/hour.

5. The method of claim 3 , wherein the target molecule comprises at least a portion of an immunoglobulin.

6. The method of claim 3 , wherein the target molecule comprises a monoclonal antibody or a polyclonal antibody.

7. The method of claim 3 , wherein the target molecule comprises at least a portion of an Fc region of an immunoglobulin.

8. The method of claim 3 , wherein the affinity ligand comprises Protein A.

9. The method of claim 3 , wherein the target molecule is eluted from the affinity chromatography matrix with a buffer having an acidic pH.

10. The method of claim 3 , wherein the affinity chromatography matrix has a dynamic binding capacity for the target molecule of at least 45 g/liter.

Assignees

• Emd Millipore Corp

Inventors

• Bian Nanying
• Ramaswamy Senthil
• Soice Neil
• Wang Chen
• Wong Yuan

Classifications

• B01J20/286

  Phases chemically bonded to a substrate, e.g. to silica or to polymers · CPC title

• B01J20/28026

  Particles within, immobilised, dispersed, entrapped in or on a matrix, e.g. a resin · CPC title

• B01J20/28016

  Particle form · CPC title

• B01J20/28085

  being more than 50 nm, i.e. macropores · CPC title

• G01N33/538

  by sorbent column, particles or resin strip {, i.e. sorbent materials} · CPC title