Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains
US-8999641-B2 · Apr 7, 2015 · US
CRISPR hybrid DNA/RNA polynucleotides and methods of use
US9580701B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9580701-B2 |
| Application number | US-201615238464-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 16, 2016 |
| Priority date | Jan 28, 2015 |
| Publication date | Feb 28, 2017 |
| Grant date | Feb 28, 2017 |
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- Title
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- Abstract
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Abstract
Official abstract text for this publication.
The present disclosure provides DNA-guided CRISPR systems; polynucleotides comprising DNA, RNA and mixtures thereof for use with CRISPR systems; and methods of use involving such polynucleotides and DNA-guided CRISPR systems.
First claim
Opening claim text (preview).
What is claimed is: 1. A single polynucleotide comprising: a targeting region that comprises deoxyribonucleic acid (DNA) or ribonucleic acid (RNA); and an activating region adjacent to said targeting region that comprises DNA; wherein said activating region comprises a stem loop structure and is capable of binding with a Cpf1. 2. The polynucleotide of claim 1 , wherein the targeting region comprises a mixture of DNA and RNA. 3. The polynucleotide of claim 1 , wherein the targeting region comprises a mixture of DNA and RNA and the activating region comprises a mixture of DNA and RNA. 4. A Class 2 CRISPR system consisting essentially of: (i) a single polynucleotide comprising (a) a targeting region that comprises deoxyribonucleic acid (DNA) or ribonucleic acid (RNA); and (b) an activating region adjacent to said targeting region that comprises DNA; wherein said activating region comprises a stem loop structure and is capable of binding with a Cpf1; and (ii) a Cpf1. 5. The Class 2 CRISPR system of claim 4 , wherein said targeting region comprises a mixture of DNA and RNA. 6. The Class 2 CRISPR system of claim 4 , wherein said activating region comprises a mixture of DNA and RNA. 7. The Class 2 CRISPR system of claim 4 , wherein said targeting region comprises a mixture of DNA and RNA and said activating region comprises a mixture of DNA and RNA. 8. The Class 2 CRISPR system of claim 4 , further comprising a donor polynucleotide.
Assignees
Inventors
Classifications
Targeted insertion of genes into the plant genome by homologous recombination · CPC title
- C12N15/907Primary
in mammalian cells · CPC title
Hydrolases acting on ester bonds (3.1) · CPC title
- C12N9/22Primary
Ribonucleases {[RNase]; Deoxyribonucleases [DNase]} · CPC title
- C12N15/111Primary
General methods applicable to biologically active non-coding nucleic acids · CPC title
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Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US9580701B2 cover?
- The present disclosure provides DNA-guided CRISPR systems; polynucleotides comprising DNA, RNA and mixtures thereof for use with CRISPR systems; and methods of use involving such polynucleotides and DNA-guided CRISPR systems.
- Who is the assignee on this patent?
- Pioneer Hi Bred Int, Pioneer Hi Bred Int
- What technology area does this patent fall under?
- Primary CPC classification C12N15/907. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 28 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 6 related publications on this page (citations in our corpus or others sharing the same primary CPC).