Apparatus for filtering fluid and methods of isolating particle using the same

Patent metadata

Field	Value
Publication number	US-9580677-B2
Application number	US-201414172576-A
Country	US
Kind code	B2
Filing date	Feb 4, 2014
Priority date	Jul 30, 2013
Publication date	Feb 28, 2017
Grant date	Feb 28, 2017

Abstract

Provided are an apparatus for filtering a fluid, the apparatus including a first flow channel and a second flow channel which are connected to each other in a fluid communicable manner via a filtration medium, and a method of isolating particles using the apparatus.

First claim

What is claimed is:

1. A method of isolating a particle from a sample using an apparatus for filtering a fluid, the apparatus comprising: a first flow channel defined by a first inlet disposed at a first end, a second inlet disposed at a second end opposite the first end, a filtration membrane having a plurality of apertures, and a wall on which the filtration membrane is disposed; and a second flow channel connected to the filtration medium in a fluid communicable manner and defined by walls and a first outlet disposed at a side opposite the filtration membrane, the method comprising:
sequentially flowing a sample comprising the particle through the first inlet, the first channel, the second channel, and the first outlet of the second channel of the apparatus for filtering a fluid; and
simultaneously with the sequentially flowing the sample, sequentially flowing a liquid medium through the second inlet of the first channel, the first channel, the second channel, and the first outlet of the second channel.

2. The method of claim 1 , wherein the flowing of the sample is performed by one or both of applying a negative pressure to the first outlet of the second channel, and applying a positive pressure to the first inlet of the first channel.

3. The method of claim 1 , wherein the flowing of the liquid medium is performed by one or both of applying a negative pressure to the first outlet of the second channel, and applying a positive pressure to the second inlet of the first channel.

4. The method of claim 2 , wherein the pressure is applied by a pump.

5. A method of isolating a particle from a sample using an apparatus for filtering a fluid, the apparatus comprising: a first flow channel defined by a first inlet disposed at a first end, a second inlet disposed at a second end opposite the first end, and a wall on which a filtration medium is disposed; and a second flow channel connected to the filtration medium in a fluid communicable manner and defined by walls and a first outlet disposed at a side opposite the filtration medium, the method comprising:
sequentially flowing a sample comprising the particle through the first inlet, the first channel, the second channel, and the first outlet of the second channel of the apparatus for filtering a fluid;
sequentially flowing a liquid medium through the second inlet of the first channel, the first channel, the second channel, and the first outlet of the second channel; and
inducing agitation of a material at the surface of the filtration medium by moving a boundary between a flow of the sample and a flow of the liquid medium with respect to the filtration medium by controlling a flow rate of the sample and a flow rate of the liquid medium.

6. The method of claim 5 , wherein a material layer formed on the surface of the filtration medium is removed by the agitation.

7. The method of claim 5 , wherein the moving of the boundary comprises a combination of moving the boundary toward the first inlet and moving the boundary toward the second inlet with respect to the surface of the filtration medium.

8. The method of claim 1 , wherein a size of the particle is greater than a diameter of each aperture.

9. The method of claim 1 , further comprising observing the flows of the sample and the liquid medium through the filtration membrane using an optical detection device,
wherein at least one portion of the first flow channel corresponding to the filtration membrane is formed of an optically clear material, and the optical detection device is disposed in the at least one portion of the first flow channel corresponding to the filtration membrane and formed of the optically clear material.

10. The method of claim 5 , further comprising observing a boundary between the flow of the sample and the flow of the liquid medium through the filtration medium by using an optical detection device,
wherein at least one portion of the first flow channel corresponding to the filtration medium is formed of an optically clear material, and the optical detection device is disposed in the at least one portion of the first flow channel corresponding to the filtration medium and formed of the optically clear material.

11. The method of claim 10 , further comprising moving the boundary between the flow of the sample and the flow of the liquid medium based on an observation result.

12. The method of claim 3 , wherein the pressure is applied by a pump.

Assignees

• Samsung Electronics Co Ltd

Inventors

• Lee Hun-Joo
• Park Jong-Myeon

Classifications

• C12N5/0602

  Vertebrate cells · CPC title

• G01N1/4077

  by other techniques involving separation of suspended solids · CPC title

• C12M47/02 Primary

  Separating microorganisms from the culture medium; Concentration of biomass (separating microorganisms from their culture media C12N1/02) · CPC title

• B01D29/904

  directing the mixture to be filtered on the filtering element in a manner to clean the filter continuously (B01D29/115, B01D29/118, B01D29/17, B01D29/25, B01D29/336, B01D29/356, B01D29/902, B01D29/908 take precedence) · CPC title

• C12M1/12 Primary

  with sterilisation, filtration or dialysis means · CPC title