Method for fractionating dextrin

Patent metadata

Field	Value
Publication number	US-9580518-B2
Application number	US-201414903240-A
Country	US
Kind code	B2
Filing date	Sep 26, 2014
Priority date	Sep 17, 2014
Publication date	Feb 28, 2017
Grant date	Feb 28, 2017

Abstract

A method for fractionating dextrin, belonging to the technical field of food processing. In principle, the method for fractionating dextrin of the present invention is based on the fact that the dextrin is incompatible with polyethylene glycol in aqueous solution and a larger molecular weight of the dextrin results in more significant incompatibility with polyethylene glycol. Accordingly, the stepwise precipitation of dextrin components having a molecular weight from large to small may be realized by gradually increasing the concentration of polyethylene glycol by gradually adding polyethylene glycol to dextrin in aqueous solution.

First claim

The invention claimed is:

1. A method for fractionating dextrin, comprising a dextrin solution preparation step and a dextrin fractionating step which employs stepwise precipitation by polyethylene glycol, specifically: adding 5 g of polyethylene glycol to 100 mL of dextrin solution at a mass concentration of 0.9%-3.6%, stirring while heating until the polyethylene glycol is dissolved, that is, the solution becomes clear, naturally cooling in air to 25° C., remaining at 25° C. in a water bath for 24 h, centrifuging for 15 min at 4000 g if any precipitation occurs, washing the obtained precipitate with trichloromethane to remove the residual polyethylene glycol, drying for 24 h at 40° C., crushing and sieving to obtain a dextrin component Grade 1; continuously adding 5 g of polyethylene glycol to the supernatant, and repeating the above processes to obtain a dextrin component Grade 2; if no precipitation occurs, continuously adding 5 g of polyethylene glycol until the precipitation occurs; and repeating the above processes until the amount of polyethylene glycol added reaches 60 g, thus to obtain 9 to 11 graded dextrin components.

2. The method for fractionating dextrin according to claim 1 , characterized in that the dextrin is obtained by the degradation of starch with alcohol-acid, specifically: dispersing 15-25 g of tapioca starch in 100 mL of n-butyl alcohol, adding 1 mL of concentrated hydrochloric acid, hydrolyzing for 3 days at 40° C., adding 14 mL of 1M NaHCO 3 to terminate the reaction, centrifuging for 15 min at 4000 g, precipitating, washing with 50% ethanol solution for several times until there is no chloride ion, drying in an oven at 40° C., crushing, and sieving to obtain the dextrin.

3. The method for fractionating dextrin according to claim 1 , characterized in that the number-average molecular weight of the used polyethylene glycol is 4000 Da, 6000 Da or 8000 Da.

Assignees

• Univ Jiangnan

Inventors

• Jin Zhengyu
• Tian Yaoqi
• Hu Xiuting
• Sun Binghua
• Zhang Bao
• Wei Benxi
• Wu Chunsen
• Yang Na
• Xu Xueming

Classifications

• C08B30/18 Primary

  Dextrin {, e.g. yellow canari, white dextrin, amylodextrin or maltodextrin; Methods of depolymerisation, e.g. by irradiation or mechanically} · CPC title