Mass spectrometer with timing determination based on a signal intensity in a chromatogram

Patent metadata

Field	Value
Publication number	US-9576780-B2
Application number	US-201114359744-A
Country	US
Kind code	B2
Filing date	Nov 22, 2011
Priority date	Nov 22, 2011
Publication date	Feb 21, 2017
Grant date	Feb 21, 2017

Abstract

A mass spectrometer including chromatogram creation means for creating a chromatogram showing changes over time in an ion intensity within a predetermined mass range based on the MS analysis results, and timing determination means for determining a timing to perform MS/MS analysis based on the chromatogram. The timing determination means determines, as a timing to perform MS/MS analysis, a point in time at which a signal intensity in the chromatogram reaches a predetermined upper limit after exceeding a predetermined lower limit or a point in time at which a signal intensity in the chromatogram reaches a top of a peak without reaching the upper limit after exceeding the lower limit. It is thus possible to collect precursor ions at a timing at which the signal intensity of a peak originating from sample components is highest between the upper limit and lower limit, thereby obtaining a high quality MS/MS spectrum.

First claim

The invention claimed is:

1. A mass spectrometer which sequentially introduces sample components which are temporally separated by a column of a chromatography into an ionization unit and performs mass analysis, comprising:
a) mass spectrometry (MS) analyzer for repeatedly performing MS analysis on ions generated in the ionization unit; and
a processor including
b) chromatogram creation unit for creating a chromatogram that shows changes over time in an ion intensity within a predetermined mass range based on a result of the MS analysis; and
c) timing determination unit for determining a timing to perform MS/MS analysis based on a signal intensity in the chromatogram;
wherein the MS analyzer performs MS/MS analysis in which ions belonging to the mass range among ions generated in the ionization unit are adopted as precursor ions, in accordance with the timing to perform MS/MS analysis that is determined by the timing determination unit, wherein:
the processor is configured to cause, when a signal intensity in the chromatogram reaches a predetermined upper limit after exceeding a predetermined lower limit, the timing determination unit to determine that point in time as the timing to perform MS/MS analysis, and when, after exceeding the lower limit, a signal intensity in the chromatogram arrives at a top of a peak without reaching the upper limit, the timing determination unit to determine that point in time as the timing to perform MS/MS analysis.

2. The mass spectrometer according to claim 1 , further comprising:
e) an operation unit for allowing a user to enter and set the upper limit and the lower limit.

3. The mass spectrometer according to claim 1 wherein:
the chromatogram creation unit creates a plurality of chromatogram relating to a predetermined plurality of mass ranges;
the processor is configured to cause, when at least any one signal intensity of the plurality of chromatograms reaches the predetermined upper limit after exceeding the predetermined lower limit, the timing determination unit to determine that point in time as the timing to perform MS/MS analysis for the respective mass range, and when the at least any one signal intensity reaches a top of a peak without reaching the upper limit after exceeding the lower limit, the timing determination unit to determine that point in time as the timing to perform MS/MS analysis for the respective mass range; and
the MS analyzer performs MS/MS analysis that, among ions generated in the ionization unit, takes ions belonging to a mass range corresponding to a chromatogram that is used by the timing determination unit as precursor ions.

4. The mass spectrometer according to claim 2 wherein:
the chromatogram creation unit creates a plurality of chromatogram relating to a predetermined plurality of mass ranges;
the processor is configured to cause, when at least any one signal intensity of the plurality of chromatograms reaches the predetermined upper limit after exceeding the predetermined lower limit, the timing determination unit to determine that point in time as the timing to perform MS/MS analysis for the respective mass range, and when the at least any one signal intensity reaches a top of a peak without reaching the upper limit after exceeding the lower limit, the timing determination unit to determine that point in time as the timing to perform MS/MS analysis for the respective mass range; and
the MS analyzer performs MS/MS analysis that, among ions generated in the ionization unit, takes ions belonging to a mass range corresponding to a chromatogram that is used by the timing determination unit as precursor ions.

Assignees

• Niwa Akihiko
• Shimadzu Corp

Inventors

• Niwa Akihiko

Classifications

• H01J49/4265

  Controlling the number of trapped ions; preventing space charge effects · CPC title

• H01J49/004

  Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn · CPC title

• H01J49/0031

  Step by step routines describing the use of the apparatus (H01J49/0081 takes precedence) · CPC title

• G01N30/8696

  Details of Software · CPC title

• G01N30/72 Primary

  Mass spectrometers {(mass spectrometers per se H01J49/00)} · CPC title