Lanthanum-based concentration agents for microorganisms

Patent metadata

Field	Value
Publication number	US-9575059-B2
Application number	US-201314400390-A
Country	US
Kind code	B2
Filing date	May 23, 2013
Priority date	Jun 5, 2012
Publication date	Feb 21, 2017
Grant date	Feb 21, 2017

Abstract

A concentration agent for microorganisms is provided that contains both lanthanum and carbonate. Additionally, articles that include the concentration agent and methods of concentrating a microorganism using the concentration agent are provided.

First claim

What is claimed is:

1. A process for concentrating a microorganism, the process comprising:
(a) providing a concentration agent comprising a plurality of particles of lanthanum/carbonate-containing materials, wherein the concentration agent has a weight ratio of carbon to lanthanum that is at least 0.05;
(b) providing a fluid sample comprising the microorganism;
(c) contacting the concentration agent with the fluid sample; and
(d) binding the microorganism to the concentration agent to form a bound microorganism.

2. The process of claim 1 , wherein the bound microorganism is in a viable state.

3. The process of claim 1 , further comprising segregating the bound microorganism from the fluid sample.

4. The process claim 1 , wherein the concentration agent further comprises a porous matrix, wherein the plurality of particles are distributed on a surface of the porous matrix, distributed throughout the porous matrix, or distributed both on the surface and throughout the porous matrix.

5. The process of claim 1 , wherein the concentration agent comprises lanthanum carbonate, lanthanum oxycarbonate, lanthanum hydroxycarbonate, or a mixture thereof.

6. The process of claim 1 , further comprising detecting the presence of the bound microorganism.

7. An article comprising:
a concentration agent comprising a plurality of particles of lanthanum/carbonate-containing materials, wherein the concentration agent has a weight ratio of carbon to lanthanum that is at least 0.05; and
a microorganism bound to the concentration agent.

8. The article of claim 7 , wherein the microorganism is in a viable state.

9. The article of claim 7 , wherein the concentration agent comprises lanthanum carbonate, lanthanum oxycarbonate, lanthanum hydroxycarbonate, or a mixture thereof.

10. An article comprising:
a concentration agent comprising a plurality of particles of lanthanum/carbonate-containing materials, wherein the concentration agent has a weight ratio of carbon to lanthanum that is at least 0.05; and
a porous matrix, wherein the concentration agent is distributed on a surface of the porous matrix, throughout the porous matrix, or a combination thereof.

11. The article of claim 10 , wherein the porous matrix comprises nonwoven fibers and an optional polymeric binder.

12. The article of claim 10 , wherein the porous matrix comprises a sintered polymeric material.

13. The article of claim 10 , wherein the porous matrix is in the form of a filter medium.

Assignees

• 3M Innovative Properties Co

Inventors

• Kshirsagar Manjiri T
• Hajime Evan Koon Lun Yuuji
• Rabins Andrew W

Classifications

• B01J20/0277

  Carbonates of compounds other than those provided for in B01J20/043 · CPC title

• G01N33/5306 Primary

  Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding · CPC title

• B01J20/28028

  Particles immobilised within fibres or filaments · CPC title

• C12Q1/24

  Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms · CPC title

• B01J20/28026

  Particles within, immobilised, dispersed, entrapped in or on a matrix, e.g. a resin · CPC title