DGAT genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants

Patent metadata

Field	Value
Publication number	US-9574207-B2
Application number	US-201414475790-A
Country	US
Kind code	B2
Filing date	Sep 3, 2014
Priority date	May 23, 2008
Publication date	Feb 21, 2017
Grant date	Feb 21, 2017

Abstract

Polynucleotide sequences encoding diacylglycerol acyltransferase genes and the use of these acyltransferases for increased seed storage lipid production and altered fatty acid profiles in oilseed plants are disclosed. Transgenic soybean seed having increased total fatty acid content of at least 20% and altered fatty acids when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. Methods for increasing the total fatty acid content of a soybean seed by at least 20% include steps of transformation and selection.

First claim

What is claimed is:

1. A transgenic soybean seed having increased total fatty acid content of at least 20% when compared to the total fatty acid content of a non-transgenic, null segregant soybean seed wherein said seed comprises at least one recombinant construct having at least one diacylglycerol acyltransferase (DGAT) sequence and a construct downregulating galactinol synthase (GAS) activity wherein the DGAT sequence and the GAS construct can be in the same recombinant construct or in separate recombinant constructs.

2. The transgenic soybean seed of claim 1 further wherein the DGAT sequence is a Yarrowia DGAT sequence.

3. A method for increasing the total fatty acid content of a soybean seed comprising:
(a) transforming at least one soybean cell with at least one recombinant construct having at least one diacylglycerol acyltransferase (DGAT) sequence and a construct downregulating galactinol synthase (GAS) activity wherein the DGAT sequence and the GAS construct can be in the same recombinant construct or in separate recombinant constructs;
(b) selecting the transformed soybean cell(s) of step (a) such that plants grown from the transformed cells produce transgenic seed having an increased total fatty acid content of at least 20% when compared to the total fatty acid content of a non-transgenic, null segregant soybean seed.

4. The method of claim 3 wherein the DGAT sequence is selected from the group consisting of DGAT1, DGAT2 and DGAT1 in combination with DGAT2.

5. The method of claim 3 or 4 further wherein the DGAT sequence is a Yarrowia sequence.

6. Progeny obtained from the transgenic soybeans of claim 1 , wherein the progeny comprise said construct or constructs.

Assignees

• Du Pont

Inventors

• Meyer Knut
• Stecca Kevin L

Classifications

• C12N9/1029

  transferring groups other than amino-acyl groups (2.3.1) · CPC title

• A23L11/07

  Soya beans, e.g. oil-extracted soya bean flakes (A23L11/30 takes precedence) · CPC title

• C12N9/1037

  Naringenin-chalcone synthase (2.3.1.74), i.e. chalcone synthase · CPC title

• C12N9/1033

  Chloramphenicol O-acetyltransferase (2.3.1.28) · CPC title

• C12N15/8247 Primary

  involving modified lipid metabolism, e.g. seed oil composition · CPC title