Regeneration and repair of neural tissue using postpartum-derived cells

Patent metadata

Field	Value
Publication number	US-9572840-B2
Application number	US-201414152649-A
Country	US
Kind code	B2
Filing date	Jan 10, 2014
Priority date	Jun 27, 2003
Publication date	Feb 21, 2017
Grant date	Feb 21, 2017

Abstract

Cells derived from postpartum umbilicus and placenta are disclosed. Pharmaceutical compositions, devices and methods for the regeneration or repair of neural tissue using the postpartum-derived cells are also disclosed.

First claim

What is claimed:

1. A method of providing factors to a patient having a neurodegenerative condition comprising administering to the patient a composition comprising a conditioned medium generated by culturing cells isolated from human umbilical cord tissue substantially free of blood, wherein said cells are capable of self-renewal and expansion in culture, have the potential to differentiate into cells of at least a neural phenotype; exhibit the ability to undergo at least about 40 doublings in culture without a change in karyotype, and lack production of CD117; and wherein the method promotes cell survival and/or neuronal differentiation and wherein the factors are selected from the group consisting of brain-derived neurotrophic factor (BDNF), interleukin-6 (IL-6), granulocyte chemotactic protein-2 (GCP-2), transforming growth factor beta2 (TGFbeta2), hepatocyte growth factor (HGF), basic fibroblast growth factor (bFGF), keratinocyte growth factor (KGF), platelet derived growth factor (PDGF-bb), heparin-binding epidermal growth factor (HB-EGF) and combinations thereof.

2. The method of claim 1 , wherein said cells produce each of CD10, CD13, CD44, CD73, CD90, PDGFr-alpha, PD-L2 and HLA-A,B,C.

3. The method of claim 1 , wherein said cells lack the production of CD31, CD34, CD45, CD80, CD86, CD141, CD178, B7-H2, HLA-G and HLA-DR,DP,DQ.

4. The method of claim 1 , wherein said cells secrete each of the factors MCP-1, IL-6, IL-8, GCP-2, HGF, KGF, FGF, HB-EGF, BDNF, TPO and TIMP1.

5. The method of claim 1 , wherein said cells do not secrete one or more of the factors SDF-1 alpha, TGF-beta2, ANG2, PDGFbb and VEGF.

6. The method of claim 1 , wherein the method promotes cell survival.

7. The method of claim 1 , wherein the method promotes neuronal differentiation.

Assignees

• Depuy Synthes Products Inc

Inventors

• Messina Darin J
• Harmon Alexander M
• Harris Ian R
• Kihm Anthony J
• Mistry Sanjay
• Seyda Agnieszka
• Yi Chin-Feng
• Gosiewska Anna

Classifications

• C12N2501/12

  Hepatocyte growth factor [HGF] · CPC title

• A61K35/12

  Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells (vaccines or medicinal preparations containing antigens or antibodies A61K39/00) · CPC title

• C12N2501/23

  Interleukins [IL] · CPC title

• C12N2502/02

  embryonic cells · CPC title

• C12N2500/95

  Protein-free medium and culture conditions · CPC title