Method for producing polymers

Patent metadata

Field	Value
Publication number	US-9568839-B2
Application number	US-40049309-A
Country	US
Kind code	B2
Filing date	Mar 9, 2009
Priority date	Feb 19, 1999
Publication date	Feb 14, 2017
Grant date	Feb 14, 2017

Abstract

The invention relates to a method for producing polymers, in particular synthetic nucleic acid double strands of optional sequence, comprising the steps:
(a) provision of a support having a surface area which contains a plurality of individual reaction areas, (b) location-resolved synthesis of nucleic acid fragments having in each case different base sequences in several of the individual reaction areas, and (c) detachment of the nucleic acid fragments from individual reaction areas.

First claim

What is claimed is:

1. A method for optimizing the functional genetic parameters of genetic elements, comprising:
(a) providing a digital sequence of a variant of a known genetic element,
(b) fragmenting the digital sequence of step (a) into a plurality of suitable complementary oligomeric building blocks that can assemble to form a nucleic acid double-stranded hybrid,
(c) synthesizing the plurality of oligomeric building blocks by parallel synthesis steps, wherein each oligomeric building block is synthesized on a different area of a common support,
(d) detaching the oligomeric building blocks from the support,
(e) bringing the oligomeric building blocks into contact with one another,
(f) linking the oligomeric building blocks enzymatically to form the variant of the known genetic element,
(g) introducing the variant directly into a target cell and observing a result, and
(h) comparing the result obtained in step (g) with a reference result of the known genetic element.

2. The method according to claim 1 , wherein the genetic elements are selected from the group consisting of genes, parts of genes, regulatory elements or viral packaging signals.

3. The method according to claim 1 , wherein the digital sequences are derived from a database.

4. Method according to claim 1 , wherein the variants are generated by random composition.

5. The method according to claim 1 , wherein the variants are generated for optimizing codon usage.

6. The method according to claim 1 , wherein in step (b) biochemical and functional parameters are taken into account for generating the variants.

Assignees

• Stähler Peer F
• Stähler Cord F
• Müller Manfred
• Synthetic Genomics Inc

Inventors

• Stähler Peer F
• Stähler Cord F
• Müller Manfred

Classifications

• B01J2219/00702

  Processes involving means for analysing and characterising the products · CPC title

• C40B50/14

  Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support · CPC title

• B01J2219/00317

  Microwell devices, i.e. having large numbers of wells · CPC title

• B01J2219/00626

  Covalent · CPC title

• B01J2219/00704

  integrated with the reactor apparatus · CPC title