Microplate reader with lid lifter for microplates

Patent metadata

Field	Value
Publication number	US-9568414-B2
Application number	US-201514697012-A
Country	US
Kind code	B2
Filing date	Apr 27, 2015
Priority date	Aug 9, 2012
Publication date	Feb 14, 2017
Grant date	Feb 14, 2017

Abstract

Microplate reader has a housing, at least one optical measuring/detection device, a microplate support, a moving unit and an integrated lid holding apparatus. The lid holding apparatus moves the microplate in one respective, at least approximately vertical direction for moving the microplate lid away from the microplate. The lid holding apparatus is arranged inside the housing as a magnetic lifter and comprises a non-array arrangement of at least one permanent magnet, or at least three electro magnets, or at least one switchable permanent magnet. Each microplate lid to be moved away from the microplate comprises magnetizable material with a physical area. A projected area of the at least one permanent magnet, electro magnet, or switchable permanent magnet is smaller than the physical area of the magnetizable material of the microplate lid.

First claim

The invention claimed is:

1. A method for optically measuring in a microplate reader ( 1 ) samples in wells ( 25 ) of a microplate ( 8 ) provided with a microplate lid ( 7 ), wherein the microplate reader ( 1 ) comprises at least one optical measuring/detection device ( 2 ), a housing ( 3 ), a microplate support ( 4 ) and a moving unit ( 5 ), wherein the moving unit ( 5 ) is arranged to move the microplate support ( 4 ) out of the housing, into the housing ( 3 ) and in at least one horizontal direction within the housing ( 3 ),
wherein the measuring method comprises the following steps:
(a) placing the microplate ( 8 ) covered by the microplate lid ( 7 ) on the microplate support ( 4 ) of the microplate reader ( 1 ), the microplate lid comprising magnetizable material;
(b) retracting the microplate support ( 4 ) and the covered microplate ( 8 ) by the moving unit ( 5 ) into the housing ( 3 ) of the microplate reader ( 1 );
(c) moving the microplate lid ( 7 ) away from the microplate ( 8 ) positioned on the microplate support ( 4 ) with a lid holding apparatus ( 6 ) integrated in the microplate reader ( 1 ) and arranged within the housing ( 3 ), and
(d) measuring samples in wells ( 25 ) of the microplate ( 8 ) with the lifted microplate lid ( 7 ) by the at least one optical measuring/detection device ( 2 ),
wherein the lid holding apparatus ( 6 ) is arranged as a magnetic lifter ( 6 ′) and comprises a non-array arrangement of at least one:
(i) permanent magnet ( 9 ′), or
(ii) electro magnet ( 10 ′), or
(iii) switchable permanent magnet ( 11 ),
and wherein each microplate lid ( 7 ) to be moved away from the microplate ( 8 ) comprises magnetizable material ( 12 ) with a physical area; a projected area of the at least one permanent magnet ( 9 ′), electro magnet ( 10 ′), or switchable permanent magnet ( 11 ) being smaller than the physical area of the magnetizable material ( 12 ) of the microplate lid ( 7 ).

2. The method of claim 1 ,
wherein a microplate lid ( 7 ) utilized for carrying out the method comprises magnetizable material ( 12 ) with a physical area that is at least double the projected area of the at least one permanent magnet ( 9 ′), electro magnet ( 10 ′), or switchable permanent magnet ( 11 ) of the magnetic lifter ( 6 ′).

3. The method of claim 1 ,
wherein moving the microplate lid ( 7 ) away from the microplate ( 8 ) is carried out by:
lifting the lid holding apparatus ( 6 ) with the microplate lid ( 7 ) in an at least approximately vertical direction away from the microplate ( 8 ), or
lowering the microplate support ( 4 ) with the microplate ( 8 ) in an at least approximately vertical direction away from the lid holding apparatus ( 6 ) with the microplate lid ( 7 ), or
lifting the lid holding apparatus ( 6 ) with the microplate lid ( 7 ) and lowering the microplate support ( 4 ) with the microplate ( 8 ) in an at least approximately vertical directions away from each other.

4. The method of claim 1 ,
wherein the microplate lid ( 7 ) is replaced on the microplate ( 8 ) positioned on the microplate support ( 4 ) by using the lid holding apparatus ( 6 ) integrated in the microplate reader ( 1 ).

5. The method of claim 1 ,
wherein the microplate support ( 4 ) and the covered microplate ( 8 ) are conveyed by the moving unit ( 5 ) out of the housing ( 3 ) of the microplate reader ( 1 ).

6. The method of claim 1 ,
wherein the microplate lid ( 7 ) is held by the lid holding apparatus ( 6 ) within the housing ( 3 ) of the microplate reader ( 1 ) or placed in the housing ( 3 ) during the optical measurement of samples in wells ( 25 ) of the microplate ( 8 ).

7. The method of claim 1 ,
wherein the samples in the wells ( 25 ) of the microplate ( 8 ) are brought into contact with air and/or a reagent for triggering luminescence or other reactions between the lifting of the microplate lid ( 7 ) from the microplate ( 8 ) positioned on the microplate support ( 4 ) according to step (c) and the optical measurement of samples in wells ( 25 ) of the microplate ( 8 ) with lifted microplate lid ( 7 ) is performed by the at least one optical measuring/detection device ( 2 ) according to step (d).

8. The curing method of claim 1 ,
wherein the microplate lid ( 7 ) is placed on the microplate ( 8 ) within the housing ( 3 ) of the microplate reader ( 1 ) by using the lid holding apparatus ( 6 ) after the optical measurement of the samples in wells ( 25 ) of the microplate ( 8 ), and the microplate support ( 4 ) and the covered microplate ( 8 ) are conveyed by the moving unit ( 5 ) out of the housing ( 3 ) of the microplate reader ( 1 ).

9. The method of claim 1 ,
wherein the microplate lid ( 7 ) is lifted and replaced several times during a long-term test, for the optical measurement of samples in wells ( 25 ) of the microplate ( 8 ) to occur.

Assignees

• Tecan Trading Ag

Inventors

• Menges Friedrich
• Gebetsroither Harald
• Wenczel Gyoergy

Classifications

• G01N2201/022

  Casings · CPC title

• G01N21/0303 Primary

  Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment (G01N21/031 - G01N21/15 take precedence) · CPC title

• G01N21/6428

  Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53) · CPC title

• G01N21/76

  Chemiluminescence; Bioluminescence · CPC title

• G01N2201/0446

  Multicell plate, sequential · CPC title