Process for producing target substance by fermentation

Patent metadata

Field	Value
Publication number	US-9567616-B2
Application number	US-201213983917-A
Country	US
Kind code	B2
Filing date	Feb 9, 2012
Priority date	Feb 9, 2011
Publication date	Feb 14, 2017
Grant date	Feb 14, 2017

Abstract

A target substance can be efficiently produced by culturing, in a medium, a coryneform bacterium in which the activity of a PTS protein relating to fructose uptake is reduced or lost as compared with a parent strain and the bacterium can produce the target substance, allowing the target substance to form and accumulate in a culture; and collecting the target substance from the culture.

First claim

The invention claimed is:

1. A process for producing a target substance, comprising:
culturing in a medium that contains glucose as a sole carbon source a coryneform bacterium in which the activity of both FruK protein and FruA protein are lost as compared with a parent strain, wherein the bacterium can produce the target substance;
allowing the target substance to form and accumulate in a culture; and
collecting the target substance from the culture, wherein
the target substance is an amino acid, a peptide or a protein.

2. The process according to claim 1 , wherein the lost activity of FruK protein and FruA protein is obtained by introducing a deletion, a substitution, or an addition of a base into genes encoding those proteins in the chromosomal DNA of the parent strain.

3. The process according to claim 1 , wherein the coryneform bacterium is Corynebacterium glutamicum.

4. The process according to claim 2 , wherein the coryneform bacterium is Corynebacterium glutamicum.

5. The process according to claim 1 , wherein the target substance is an amino acid selected from the group consisting of L-lysine, L-arginine, L-histidine, L-isoleucine, L-valine, L-leucine, L-threonine, L-phenylalanine, L-tyrosine, L-tryptophan, L-cysteine, L-glutamic acid, L-citrulline, L-glutamine, L-proline, L-serine, L-ornithine, L-methionine, L-aspartic acid, L-asparagine, and glycine.

6. The process according to claim 2 , wherein the target substance is an amino acid selected from the group consisting of L-lysine, L-arginine, L-histidine, L-isoleucine, L-valine, L-leucine, L-threonine, L-phenylalanine, L-tyrosine, L-tryptophan, L-cysteine, L-glutamic acid, L-citrulline, L-glutamine, L-proline, L-serine, L-ornithine, L-methionine, L-aspartic acid, L-asparagine, and glycine.

7. The process according to claim 3 , wherein the target substance is an amino acid selected from the group consisting of L-lysine, L-arginine, L-histidine, L-isoleucine, L-valine, L-leucine, L-threonine, L-phenylalanine, L-tyrosine, L-tryptophan, L-cysteine, L-glutamic acid, L-citrulline, L-glutamine, L-proline, L-serine, L-ornithine, L-methionine, L-aspartic acid, L-asparagine, and glycine.

8. The process according to claim 4 , wherein the target substance is an amino acid selected from the group consisting of L-lysine, L-arginine, L-histidine, L-isoleucine, L-valine, L-leucine, L-threonine, L-phenylalanine, L-tyrosine, L-tryptophan, L-cysteine, L-glutamic acid, L-citrulline, L-glutamine, L-proline, L-serine, L-ornithine, L-methionine, L-aspartic acid, L-asparagine, and glycine.

Assignees

• Ujihara Tetsuro
• Abe Tetsuya
• Yagasaki Makoto
• Kyowa Hakko Bio Co Ltd

Inventors

• Ujihara Tetsuro
• Abe Tetsuya
• Yagasaki Makoto

Classifications

• C12Y207/01069

  Protein-Npi-phosphohistidine-sugar phosphotransferase (2.7.1.69), i.e. sucrose phosphotransferase system II · CPC title

• C12P13/04

  Alpha- or beta- amino acids {(other amino acids C12P13/005)} · CPC title

• C12N9/1205

  Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases · CPC title

• Y02P20/52

  using catalysts, e.g. selective catalysts · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title