Combined use of CRY1Ca and CRY1Fa proteins for insect resistance management

Patent metadata

Field	Value
Publication number	US-9567602-B2
Application number	US-201013516611-A
Country	US
Kind code	B2
Filing date	Dec 16, 2010
Priority date	Dec 16, 2009
Publication date	Feb 14, 2017
Grant date	Feb 14, 2017

Abstract

The subject invention includes methods and plants for controlling lepidopteran insects. The plants comprise a Cry1Fa insecticidal protein and a Cry1Ca insecticidal protein, in combination, to delay or prevent development of resistance by the insect(s).

First claim

We claim:

1. A transgenic plant comprising a DNA encoding the Cry1Ca insecticidal protein of SEQ ID NO:5 and a DNA encoding the Cry1Fa insecticidal protein of SEQ ID NO:4.

2. A seed of the plant of claim 1 wherein the seed comprises said DNA encoding said Cry1Ca insecticidal protein and said DNA encoding said Cry1Fa insecticidal protein.

3. A population of plants comprising non-Bt refuge plants and a plurality of the transgenic plants of claim 1 , wherein said refuge plants comprise less than 40% of said population of plants.

4. The population of plants of claim 3 , wherein said refuge plants comprise less than 5% of said population of plants.

5. A mixture of seeds comprising refuge seeds from non-Bt refuge plants, and a plurality of seeds of claim 2 , wherein said refuge seeds comprise less than 40% of all the seeds in the mixture.

6. The mixture of seeds of claim 5 , wherein said refuge seeds comprise less than 5% of all the seeds in the mixture.

7. The transgenic plant of claim 1 , said plant further comprising DNA encoding a Cry1Ab core toxin-containing protein.

8. A of plants comprising non-Bt refuge plants and a plurality of transgenic plants of claim 7 , wherein said refuge plants comprise less than about 20% of said population of plants.

9. The population of plants of claim 8 , wherein said refuge plants comprise less than about 10% of said population of plants.

10. A composition for controlling or preventing Cry-resistant Spodoptera frugiperda insects, said composition comprising cells that express insecticidally c active amounts of both the Cry1Fa core toxin-containing protein of SEQ ID NO: 4 and the Cry1Ca core toxin-containing protein of SEQ ID NO:5.

11. The composition of claim 10 , wherein said cell is a microorganism or a plant cell.

12. The transgenic plant of claim 1 , wherein said plant a third protein selected from the group consisting of Vip3A, Cry1D, Cry1Be, and Cry1E proteins.

13. A population of plants comprising non-Bt refuge plants and a plurality of transgenic plants of claim 12 , wherein said refuge plants comprise less than about 10% said population to plants.

14. The population of plants of claim 13 , wherein said refuge plants comprise less than about 5% of said population of plants.

15. A mixture of seeds comprising refuge seeds from non-Bt refuge plants and a plurality of seeds from the plant of claim 12 , wherein said refuge seeds comprise less than 10% of all the seeds in the mixture.

16. The plant of claim 1 , wherein said plant is selected from the group consisting of corn, soybeans, and cotton.

17. The plant of claim 16 , wherein said plant is a corn plant.

Assignees

• Meade Thomas
• Narva Kenneth
• Storer Nicholas P
• Sheets Joel J
• Woosley Aaron T
• Burton Stephanie L
• Dow Agrosciences Llc

Inventors

• Meade Thomas
• Narva Kenneth
• Storer Nicholas P
• Sheets Joel J
• Woosley Aaron T
• Burton Stephanie L

Classifications

• C12N15/8286 Primary

  for insect resistance · CPC title

• A01N2300/00

  Combinations or mixtures of active ingredients covered by classes A01N27/00 - A01N65/48 with other active or formulation relevant ingredients, e.g. specific carrier materials or surfactants, covered by classes A01N25/00 - A01N65/48 · CPC title

• A01N65/00

  Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof (containing compounds of determined constitution A01N27/00 - A01N59/00) · CPC title

• A01N63/02

  Human Necessities · mapped topic

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title