Polypeptides having glucoamylase activity and polynucleotides encoding same

Patent metadata

Field	Value
Publication number	US-9567574-B2
Application number	US-201214343911-A
Country	US
Kind code	B2
Filing date	Sep 7, 2012
Priority date	Sep 9, 2011
Publication date	Feb 14, 2017
Grant date	Feb 14, 2017

Abstract

The present invention provides isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. The invention also provides nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.

First claim

The invention claimed is:

1. A process of producing a fermentation product from starch-containing material comprising the steps of:
(a) liquefying starch-containing material;
(b) saccharifying the liquefied material; and
(c) fermenting with a fermenting organism;

wherein step (b) is carried out by contacting the liquefied material with at least a glucoamylase having at least 85% sequence identity to the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4, and wherein the fermentation product is ethanol.

2. The process of claim 1 wherein the glucoamylase has at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 2.

3. The process of claim 1 wherein the glucoamylase has at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2.

4. The process of claim 1 wherein the glucoamylase has at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 2.

5. The process of claim 1 wherein the glucoamylase has at least 98% sequence identity to the mature polypeptide of SEQ ID NO: 2.

6. The process of claim 1 wherein the glucoamylase has at least 99% sequence identity to the mature polypeptide of SEQ ID NO: 2.

7. The process of claim 1 wherein the glucoamylase has at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 4.

8. The process of claim 1 wherein the glucoamylase has at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 4.

9. The process of claim 1 wherein the glucoamylase has at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 4.

10. The process of claim 1 wherein the glucoamylase has at least 98% sequence identity to the mature polypeptide of SEQ ID NO: 4.

11. The process of claim 1 wherein the glucoamylase has at least 99% sequence identity to the mature polypeptide of SEQ ID NO: 4.

12. The process of claim 1 , wherein the fermenting organism is yeast.

13. A process of producing a fermentation product from starch-containing material comprising the steps of:
(a) saccharifying starch-containing material at a temperature below the initial gelatinization temperature of said starch-containing material; and
(c) fermenting with a fermenting organism;

wherein step (a) is carried out by contacting the starch-containing material with at least a glucoamylase having at least 85% sequence identity to the mature polypeptide of SEQ ID NO: 2 or SEQ ID NO: 4, and wherein the fermentation product is ethanol.

14. The process of claim 13 wherein the polypeptide has at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 2.

15. The process of claim 13 wherein the polypeptide has at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2.

16. The process of claim 13 wherein the polypeptide has at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 2.

17. The process of claim 13 wherein the polypeptide has at least 98% sequence identity to the mature polypeptide of SEQ ID NO: 2.

18. The process of claim 13 wherein the polypeptide has at least 99% sequence identity to the mature polypeptide of SEQ ID NO: 2.

19. The process of claim 13 wherein the polypeptide has at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 4.

20. The process of claim 13 wherein the polypeptide has at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 4.

21. The process of claim 13 wherein the polypeptide has at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 4.

22. The process of claim 13 wherein the polypeptide has at least 98% sequence identity to the mature polypeptide of SEQ ID NO: 4.

23. The process of claim 13 wherein the polypeptide has at least 99% sequence identity to the mature polypeptide of SEQ ID NO: 4.

24. The process of claim 13 , wherein the fermenting organism is yeast.

Assignees

• Sun tianqi
• Li Ming
• Novozymes As

Inventors

• Sun tianqi
• Li Ming

Classifications

• C12P2203/00

  Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source · CPC title

• C12N9/2428 Primary

  Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase · CPC title

• C12P19/02

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title

• C12P7/14

  Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms · CPC title

• C13K1/06

  obtained by saccharification of starch or raw materials containing starch · CPC title