Array domains having rotated patterns

Patent metadata

Field	Value
Publication number	US-9566560-B2
Application number	US-201113267565-A
Country	US
Kind code	B2
Filing date	Oct 6, 2011
Priority date	Oct 6, 2011
Publication date	Feb 14, 2017
Grant date	Feb 14, 2017

Abstract

An article of manufacture having a plurality of sites in domains of regular patterns. Neighboring domains are oriented at different angles to improve the identification of the sites.

First claim

The invention claimed is:

1. An article of manufacture, comprising:
(a) a substrate having a surface
(b) a plurality of sites comprising one or more chemical moieties or biological molecules disposed at respective discrete locations on the surface and organized in at least one sample area, each sample area comprising at least two neighboring domains
(c) a first domain of the at least two neighboring domains having a first layout comprising sites in a regular pattern and
(d) a second domain of the at least two neighboring domains having a second layout comprising sites in the regular pattern, the regular pattern being oriented differently in the second layout relative to the first layout.

2. The article of claim 1 , comprising at least two sample areas.

3. The article of claim 1 , wherein the domains of a sample area are arranged in rows and columns.

4. The article of claim 1 , wherein each domain comprises at least 1000 sites.

5. The article of claim 1 , wherein no sites are disposed in the areas between neighboring domains.

6. The article of claim 1 , wherein the regular pattern comprises a rectangular pattern.

7. The article of claim 1 , wherein the regular pattern comprises a triangular or pentagonal pattern.

8. The article of claim 1 , wherein the regular pattern comprises a hexagonal pattern.

9. The article of claim 1 , wherein the regular pattern in a domain exhibits at least one angle interval of rotational symmetry, and the angles of neighboring domains differ from each other by an amount that is not equal to an angle interval.

10. The article of claim 1 , wherein the second layout is angularly-displaced by about 45 degrees with respect to the first layout.

11. The article of claim 1 , having four different layouts.

12. The article of claim 11 , wherein the layouts are angularly-displaced by about 15, about 30, and about 45 degrees with respect to each another.

13. The article of claim 1 , wherein the sites contain chemical groups.

14. The article of claim 1 , wherein the minimum distance between any two sites is at most 2 microns.

15. The article of claim 1 , wherein a plurality of sites are wells.

16. The article of claim 15 , wherein the wells are at most 1.2 microns across.

17. The article of claim 1 , wherein a plurality of sites support a material of interest.

18. The article of claim 17 , wherein the material is a biological molecule.

19. The article of claim 17 , wherein, within a single sample area, the sites support a plurality of different materials.

20. The article of claim 19 , wherein each sample area supports greater than 1000 different materials.

21. The article of claim 1 , wherein each individual site within the regular pattern comprises a plurality of molecules of a single type.

22. The article of claim 1 , comprising a plurality of different biopolymers such that the plurality of sites of the regular pattern have different attached biopolymers relative to one another.

23. The article of claim 1 , wherein each individual site within the pattern comprises at least one bead.

24. The article of claim 1 , wherein each individual site has a diameter of 1.2 microns.

25. The article of claim 1 , wherein the plurality of sites are separated by a uniform pitch.

Assignees

• Harris Jason
• Tanner Stephen
• Illumina Inc

Inventors

• Harris Jason
• Tanner Stephen

Classifications

• C12Q1/6837 Primary

  using probe arrays or probe chips (C12Q1/6874 takes precedence) · CPC title

• B01J19/0046 Primary

  Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays (synthesis methods per se C40B50/00) · CPC title

• B01J2219/00693

  Means for quality control · CPC title

• B01J2219/00608

  DNA chips · CPC title

• B01J2219/00662

  Two-dimensional arrays within two-dimensional arrays · CPC title